胆道闭锁患儿肝脏组织c-kit+细胞的表达及意义

目的探讨胆道闭锁患儿肝脏组织c-kit+细胞表达及其与肝脏纤维化程度与预后的关系.方法20例胆道闭锁患儿肝脏组织标本,应用免疫组化PV6000二步法染色检测c-kit+细胞.结合常规病理检查进行纤维化程度分级.根据临床资料将患儿分为预后良好组与预后不良组,并取非肝胆疾病新生儿肝脏组织作为正常对照.结果胆道闭锁患儿c-kit+细胞数显著多于正常对照组(P＜0.01);预后不良组c-kit+细胞数显著多于预后良好组(P＜0.05).纤维化程度较重组c-kit+细胞数显著多于纤维化程度较轻组(P＜0.01).结论c-kit+细胞参与胆道闭锁患儿肝脏损害、纤维化等病理过程,c-kit+细胞过度表达与胆道闭锁患儿肝脏的纤维化程度及预后密切相关.

Expression and significance of c-kit+ cells in the liver of patients with biliary atresia

Objective To assess the c-kit + cells expression of the liver in patients with biliary atresia ,and to search the relationship among the c-kit + cells expression of the liver ,the degree of liver fibrosis and the prognosis of patients with biliary atresia.Methods The c-kit + cells expression of the liver in patients with biliary atresia were assayed by Immunohistochemical PV 6000 two-step methods.20 patients were divided into two groups (good and poor outcome groups) according to their outcome of hepatic portoenterostomy. Five liver samples of normal infants were served as controls.Results The degree of c-kit + cells expression in patients with biliary atresia was higher than that of in controls; higher in poor out-come group than that of in good outcome group; higher in more severe fibrosis group than that of in more mild fibrosis group.Conclusions C-kit + cells may play an important role in the procession of liver damage and fibrosis in patients with biliary atresia; The overexpression of c-kit in the liver of patients with biliary atresia indicates that the degree of liver fibrosis is more severe and the prognosis of patients with biliary atresia is more poor.