植物抗氧化剂TA9901保护臂丛撕脱后运动神经元的实验研究

目的　观察植物抗氧化剂TA990 1对臂丛撕脱后脊髓运动神经元c jun基因的表达和存活的影响。方法　成年SD雌性大鼠 90只分成 2组 ,治疗组行右侧臂丛神经根撕脱术后 ,每天腹腔注射质量浓度为 0 .5%的TA990 1溶液 1ml,对照组注射生理盐水。治疗后 4h～ 6周处死动物行c jun免疫组织化学和中性红染色 ,定量比较两组大鼠损伤侧c jun基因表达阳性和存活运动神经元数目。结果　治疗后 3d ,5d ,1周和 2周 ,治疗组的c jun基因表达阳性运动神经元数目均多于对照组 ,两组间各时间点的差异均具有非常显著意义 (P <0 .0 1)。治疗后 2、4、6周 ,治疗组存活运动神经元数目多于对照组 ,两组间的差异均有非常显著意义 (P <0 .0 1)。结论　TA990 1能增强受损运动神经元c jun基因的表达 ,提高臂丛撕脱后运动神经元的存活率

Experimental study of botanic antioxidant TA9901 protection of rat spinal motoneurons in brachial plexus root avulsions

ObjectiveTo observe the effect of botanic antioxidant TA9901 o n injured motoneurons following brachial plexus root avulsion. MethodsBrachial p lexus root avulsion model was made in 90 adult Sprague-Dawley female rats. Then the animals were divided into 2 groups, with 45 each. The experiment group recei ved 1ml intraperitoneal injection of 0.5% TA9901 (TA9901 group) while the contro l group received normal saline everyday. The rats were killed after antioxidant treatment for 4 hours to 6 weeks. The C7 segments were examined with c-jun immu n ohistochemistry and neutral red stain. The numbers of c-jun positive and surviv a l motoneurons in the lesion side were counted and compared between the two group s. ResultsThe number of c-jun positive motoneurons was signif icantly increased in TA9901 group than that in control group at 3 days, 5 days, 1 week, and 2 w eeks respectively. The number of surviving motoneurons was also significantly in creased in TA9901 group than that in control group at 2 weeks, 4 weeks, and 6 w eeks respectively. ConclusionTA9901 can up-regulate c-jun ex pression and enha nce the survival of spinal motoneurons following brachial plexus root avulsion.