内皮细胞源性IL—8对人树突状细胞作用的研究

目的：探讨内皮源性白细胞介素－８（ＩＬ－８）对人树突状细胞（ＤＣ）的作用。方法：用ＴＮＦ－α分别诱导内皮细胞和单核细胞产生ＩＬ－８，并将这２种不同来源的ＩＬ－８分别加入ＤＣ常规诱导培养的早期（第３天）及后期（第７天），培养至第９天收获细胞。采用流式细胞术（ＦＣＭ）分析ＤＣ的表型；体外混合淋巴细胞反应（ＭＬＲ）测定ＤＣ刺激Ｔ细胞增殖能力；ＥＬＩＳＡ检测培养上清中ＩＬ－１２的含量；ＰＩ染色观察ＤＣ凋亡。结果：在ＤＣ培养的早期（第３天）加入内皮源性ＩＬ－８可以抑制ＤＣ的成熟，ＦＣＭ分析表明：ＣＤ１ａ，ＣＤ４０，ＣＤ８０，ＣＤ８３，ＨＬＡ－ＤＲ等ＤＣ表面标志显著下降，而ＣＤ１４表达率明显高于常规培养组。同时，激发同种Ｔ细胞增殖的能力及分泌的ＩＬ－１２量均显著低于常规组（Ｐ〈０．０１），而加入单核细胞来源的ＩＬ－８则无此

The negative impact on human dendritic cells: role of endothelial interleukin-8

Objective: To explore the impact of endothelial interleukin-8(IL-8) o n human dendritic cells. Methods: Human endothelial cells and monocytes can prod uce and secrete IL-8 respectively by stimulation of TNF-α. These two types o f I L-8 of different origins were added to the routine DC culture medium at an earl y stage (3rd day) or at a late stage (7th day) mataining till cells were collecte d at 9th day. Cell differentiation and function were evaluated by flow cytome try(FCM) , mixed-lymphocyte reaction(MLR), detection of IL-12 of supernernt by using EL IS A. Apoptosis was observed by PI staining.Results: Endothelial IL-8 which if adde d at an early stage (3rd day) to DC culture could inhibit DC maturation and ther e showed greatly decreased levels DC markers:CD1a,CD40,CD80,CD83 and HLA-DR, whil e with CD14 expression obviously up-regulated. This inhibitory effects were als o demonstrated in a reduced allogenenic MLR and IL-12 secretion. Monocytic IL-8 do es not have such negative impact on DCs. Neither endothelial IL-8 nor monocyti c IL-8 would induce DC apoptosis.Conclusion: Endothelial IL-8 added to DC culture at an early stage could inhibit DC maturation and may conduct the macrophage li neage differentiation.