母体暴露壬基酚对仔鼠脾脏组织的氧化损伤

目的]研究母体暴露壬基酚（NP）对仔鼠脾脏的氧化损伤。方法]将SD母鼠随机分为50、100、200mg／kgNP暴露组及对照组，每组6～7只动物。在母鼠妊娠第9～15天灌胃NP，仔鼠（F1）于出生印天龄时处死并取脾脏称重。比色法测定脾脏组织中超氧化物歧化酶（SOD）活力及脂质过氧化产物丙二醛（MDA）含量；透射电镜观察其超微结构改变。结果]与对照组比较，200mg／kgNP组仔鼠的脾脏重量及脏器系数均明显降低（P〈0．05），且与染毒剂量呈负相关（r脾=-0．618，r脏=-0．530，P〈0．05）；100、200mg／kgNP组仔鼠脾脏组织的SOD活力降低，200mg／kgNP组MDA含量升高（P〈0．05），均存在剂量效应关系（rSOD=-0．460，rMnA=0．747，P〈0．05）。高暴露组仔鼠脾脏电镜下可见脾细胞线粒体肿胀呈空泡样变，染色质浓缩成块状聚集在核周围。结论]NP可透过胎盘屏障致仔鼠脾脏脂质过氧化损伤。

Toxicity of Nonylphenol on F1 Rats' Spleen after Gestation Exposure

Objective ] To investigate the adverse effects on F] rats＇spleen while the pregnant mother rats were exposed to nonylphenol （ NP ）. Methods ] Pregnant rats were exposed to NP via gavage at doses of 0, 50, 100 and 200 mg/kg by body weights respectively per day, from pregnant days 9 to 15. The level of malondialdehyde（ MDA ）and activity of super oxide dimutese （ SOD ） in FI rats＇ spleen homogenate were detected respectively at the age of 60th day. The ultrastructure of spleen tissues were observed under electronic microscope. Results ] Compared with the control group, the absolute and relative weight of spleen were decreased dramatically in the 200 mg/kg NP treated group and negative relation between the weight and dose was found （ rSOD=-0.618, rcoefficient=-0.530, P〈0.05 ）. The SOD activity of spleen tissues decreased in NP I00 and 200 mg/kg exposed fetal rats, while the levels of MDA increased in NP 200mg/kg exposed fetal rats. Both of them were in a dose-dependent manner （rSOD=-0.460, rMDA=0.747, P〈0.05 ）. The differences mentioned above were statistical significant compared to control group（ P〈0.05 ）. The changes of the uhrastructrue were found among the spleen of NP 200 mg/kg group that characterized with ehromatin condesed, clumped in circa-nuclei and mitochondrial tumefaction and vaeuolization. Conclusion ] NP can get across the placenta barrier to make the F1 offspring rats＇spleen damage of lipid peroxidation.