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Effects of skin storage conditions and concentration of applied dose on [3H]T-2 toxin penetration through excised human and monkey skin
Authors:B W Kemppainen  R T Riley  J G Pace  F J Hoerr
Affiliation:1. Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria 3052, Australia;2. Laboratorio de Virologia Aplicada, Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, Florianópolis-SC 88040-900, Brazil;1. Department of Mechanical Engineering, Stanford University, 452 Panama St Building 520, Stanford, CA, 94305, USA;2. Department of Environmental Science, University of San Francisco, 2130 Fulton street, San Francisco, CA 94117, USA;3. Department of Civil and Environmental Engineering, Stanford University, 473 Via Ortega, Stanford, CA, 94305, USA;4. Institute for Computational and Mathematical Engineering, Jen-Hsun Huang Engineering Center, Stanford University, 473 Via Ortega, Stanford, CA, 94305, USA;1. School of Chemical Engineering, Shaanxi Key Laboratory of Degradable Medical Material, Northwest University, Xi''an 710069, Shaanxi, China;2. Department of Chemistry, Hong Kong Baptist University, Waterloo Road, Kowloon Tong, Hong Kong, China;3. Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, TX 78712-0165, United States
Abstract:Penetration of [3H]T-2 toxin through excised human and monkey skin stored at -60 degrees C was faster than through human and monkey skin stored at 4 degrees C, respectively. The permeability of refrigerated human skin was 34% of the permeability of refrigerated monkey skin. Increasing the concentration of [3H]T-2 toxin applied to the refrigerated monkey skin increased the amount of [3H]T-2 toxin penetrating the skin and enhanced the efficiency of penetration. Metabolites of [3H]T-2 toxin were identified in the receptor fluid bathing the dermal side of the excised human and monkey skin.
Keywords:
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