HPLC法同时测定养胃舒颗粒中绿原酸、牡荆素鼠李糖苷及橙皮苷的含量

目的：建立HPLC法同时测定养胃舒颗粒中绿原酸、牡荆素鼠李糖苷和橙皮苷3个成分的方法.方法：色谱条件：Agilent Zorbax SB-C18（250 mm×4.6 mm,5 μm）色谱柱,以乙腈 （A）-0.1% 甲酸 （B） 体系为流动相进行梯度洗脱,流速为1.0 ml·min-1,柱温为30℃,检测波长为330 nm.结果：3种成分均能达到基线分离,绿原酸、牡荆素鼠李糖苷和橙皮苷的进样量分别在0.020～0.403 μg（r=0.999 7）、0.022～0.450 μg（r=0.999 6）和0.009～0.189 μg（r=1.000 0）内与峰面积呈良好的线性关系（n=6）,平均加样回收率在95%～105%（n=6）.结论：该方法简便、快速、可靠、重复性好,可为养胃舒颗粒的质量控制提供参考依据.

Simultaneous Determination of Vitexin Rhamnoside, Chlorogenic Acid and Hesperidin in Yangweishu Granules by HPLC

Objective： To establish an HPLC method for the simultaneous determination of chlorogenic acid, vitexin rhamnoside and hesperidin in Yangweishu granules. Methods： The HPLC separation was performed on a Zorbax SB-C18 （250 mm × 4.6 mm, 5 μm） column using acetonitrile （ A ） -0.1% formic acid （B） as the mobile phase with gradient elution at the flow rate of 1. 0 ml·min-1. The column temperature was at 30℃ and the detection wavelength was 327nm. Results： The results showed that the three ef- fective components were separated well and had good linear relationship ： chlorogenic acid was within the range of 0.020-0. 403 μg（ r = 0. 999 7）, vitexin rhamnoside was within the range of 0. 022-0. 450μg （ r = 0. 999 6） and hesperidin was within the range of 0. 009- 0.189μg （ r = 1. 000 0, n = 6 ）. The average recovery was between 95 % - 105 % （ n = 6 ）. Conclusion： The method is steady with high precision and good repeatability. It can be used in the determination of active ingredients and quality control of Yangweishu granules.