星点设计-效应面法优选小儿健脾膏的提取工艺

目的：优选小儿健脾膏的提取工艺。方法：以橙皮苷提取量和浸膏得率的总评“归一值”为因变量，加水量、提取时间为自变量，采用星点设计二因素五水平表安排试验，进行二次多项式拟合，描绘三维效应面，通过效应面法选取提取工艺并进行预测分析。结果：最佳工艺条件为加21倍量水煎煮2次，每次160 min；橙皮苷平均提取量13.245 mg·g^-1，平均浸膏得率42.37%，总评“归一值”预测值与理论值的偏差率1.429%。结论：优选的提取工艺稳定可行，可作为小儿健脾膏的生产工艺。

Optimization of Extraction Technology for Children Jianpi Ointments by Central Composite Design-Response Surface Methodology

Objective: To optimize extraction process of children Jianpi ointments by central composite design-response surface methodology. Method: Extraction technology of children Jianpi ointments was optimized by taking water volume and extraction time as independent variables,overall desirability value of extract yield and the content of hesperidin as evaluation index;Central composite design was adopted to design a two factors-five levels table and conduct test-screening,then quadratic polynomial fitting and depicting of 3D-response surface were performed,extraction process was selected by response surface methodology. Result: Optimal extraction technology was as follows:extracted twice with 21-folds of water,160 min per time.Under these conditions,average yield of hesperidin was 13.245 mg·g^-1,average extract yield was 42.37%,bias between the observed and predicted value was 1.429%. Conclusion: This optimized extraction technology was stable and feasible,which could be as production process of children Jianpi ointments.