清络通痹方中雷公藤配伍减毒的化学基础研究

目的：通过研究清络通痹方中雷公藤配伍其他药味后，雷公藤中毒性成分雷公藤甲素的溶出规律，从化学角度探索其配伍减毒的物质基础，为雷公藤的临床应用提供实验依据。方法：结合“组分剔除法”、“药味组合法”，采用高效液相色谱法测定清络通痹全方、雷公藤各配伍组合中雷公藤甲素溶出含量。在此基础上，进一步对雷公藤药材、雷公藤单味药、雷公藤配伍三七、雷公藤配伍青风藤进行化学成分的全成分分析，比较雷公藤中雷公藤甲素等成分的溶出变化。结果：在雷公藤投料量相同的情况下，雷公藤甲素溶出的趋势为：全方去三七>全方去青风藤 >全方去地黄 >全方去僵蚕 >全方；青风藤配伍雷公藤 >单味雷公藤 >地黄配伍雷公藤 >僵蚕配伍雷公藤 >三七配伍雷公藤；统计分析表明，三七能明显降低雷公藤甲素溶出（P<0.05），青风藤能显著增加雷公藤甲素溶出（P<0.05）。全成分分析研究中，雷公藤配伍三七，配伍青风藤后与雷公藤药材及单味药提取物相比，溶出规律与前述结果一致。结论：通过清络通痹方中雷公藤配伍前后化学成分的溶出含量变化可以初步推断，三七配伍雷公藤能降低雷公藤的毒性，而青风藤配伍雷公藤能增强雷公藤的毒性。该研究结果为雷公藤的临床配伍减毒应用提供了实验依据。

Chemical Basisof Tripterygium wilfordii Compatibility for Attenuation in Qingluo Tongbi Decoction

Objective: Study on the regularity oftriptolidedissolution in Qingluo Tongbi decoction to find the chemical basis related to Tripterygium wilfordii compatibility for attenuation. Method: Concentrations of triptolide in Qingluo Tongbi decoction and T. wilfordii combination groups were determined by HPLC binding component-knockout and forluma combination approach.Total components in T. wilfordii, T. wilfordii decoction, combined application of T. wilfordi and Notoginseng Radix et Rhizoma as well as T. wilfordii and Sinomenii Caulis were analyzed to find the dissolution difference of marjor elements between the combination groups. Result: The concentrations of triptolide werein the order under the same dose of T. wilfordii: the whole recipe exclude Notoginseng Radix et Rhizoma> the whole recipe exclude Sinomenii Caulis> whole recipe exclude Rehmanniae Radix> the whole recipe exclude Bombyx Batryticatus> the whole recipe; T. wilfordii and Sinomenii Caulis> T. wilfordii> T. wilfordii and Rehmanniae Radix> T. wilfordii and Bombyx Batryticatus> T. wilfordii and Radix Notoginseng et Rhizoma. The statistics analysis showed that Notoginseng Radix et Rhizoma significantly reduced the dissolving action of triptolide (P<0.05) while Sinomenii Caulis promoted its dissolution(P<0.05). The dissolubility rules of T. wilfordii in different groups based on total components analysis were in accord with that above. Conclusion: Based on the dissolution changes of chemical components of T. wilfordii due to compatibility in Qingluo Tongbi decoction, it could be preliminarily inferred that T. wilfordii induced toxicity could be decreased by combination of T. wilfordii and Notoginseng Radix et Rhizoma while increased when T. wilfordii and Sinomenii Caulis were used simultaneously, which provided exprimental basis of T. wilfordii combinations for attenuationin clinical therapy.