维药芜菁籽多糖体外抗氧化活性研究

目的：通过体外清除自由基试验初步研究芜菁籽多糖的抗氧化活性。方法：采用脱脂，水提，除蛋白，脱色，醇沉等方法提取多糖。利用4种常见的体外抗氧化活性测试方法，即清除DPPH·自由基，羟自由基，超氧阴离子自由基以及总还原能力等来衡量芜菁籽多糖的抗氧化能力，并与维生素C（Vc）进行对照。结果：芜菁籽多糖提取率为2.34%，并具有一定的抗氧化活性，抗氧化能力与浓度之间有很好的量效关系，浓度越大，抗氧化活性越强。其中芜菁籽多糖的总还原能力较强，清除超氧阴离子自由基的能力较弱。结论：芜菁籽多糖具有天然抗氧化能力，在以芜菁籽为原料开发多糖类功能食品方面有较现实的实际意义。

Study on Antioxidative Activity of Polysaccharide from Uighur Medicine Turnip Seed in vitro

Objective: An preliminary study on antioxidant activity of Turnip seed polysaccharide was carried out through radical scavenging experiment in vitro. Method: Several methods include degreasation, water extraction, deproteinization, decolorization and alcohol precipitation were adopted to extract polysaccharide. Four common of antioxidant activity in vitro testing methods were selected,namely scavenging DPPH radical, hydroxyl radical, superoxide anion radical and total reducing ability, to measure the antioxidant capacity of Turnip seed polysaccharide, and ascorbic acid Vit C(Vc) was selected as the positive control. Result: Turnip seed polysaccharide have a certain amount of antioxidant activity,and the extraction rate of is 2.34%. The antioxidant capacity of polysaccharide and its concentration have good dose-effect relationship. Besides, higher concentration can enhance the antioxidant activity. Meanwhile, Turnip seed polysaccharide has strong total reduction ability, but relatively weak superoxide anion radical scavenging capacity. Conclusion: The experiments show that Turnip seed polysaccharide has natural antioxidant capacity, and using turnip seed as raw materials to develop multi-functional carbohydrate foods will have a more realistic practical significance.