多株幽门螺杆菌肽脱甲酰基酶基因克隆及序列比较

目的为开发以幽门螺杆菌(Hp)肽脱甲酰基酶为靶位的新药，首先考察该基因的保守性。方法从我国不同地区分离、选择多株Hp，培养并提取细菌DNA，设计特异引物以PCR方法扩增其肽脱甲酰基酶基因(def)，并克隆到pGEM T-Easy载体中，转化大肠杆菌，提取质粒鉴定重组质粒并测序。结果经序列比较，发现def基因有高度保守性，在重要的基序点未发生变异。结论本研究为以肽脱甲酰基酶作为靶位筛选药物提供了依据。

Cloning and sequence alignment of peptide deformylases from various strains of Helicobacter pylori

Objective To verify gene conservation of peptide deformylases (PDF) from H.pylori . Methods Ten strains from different regions of China were isolated and used to clone this gene. Bacterial DNA was extracted and used as a template to amplify def gene by PCR. def genes obtained were subsequently cloned into pGEM T easy vector and recombinants were identified by enzyme digestion. Results Sequence alignment shows that this gene is extremely conserved in H.pylori . There is no variation in three important motifs related to its function. Conclusion PDF may be an ideal target for new drug development.