Enhancement of PDGF-BB mitogenic activity on human dermal fibroblasts by biospecific dextran derivatives |
| |
Authors: | Vercoutter-Edouart Anne-Sophie Dubreucq Guy Vanhoecke Barbara Rigaut Christelle Renaux François Dahri-Correia Latifa Lemoine Jérôme Bracke Marc Michalski Jean-Claude Correia José |
| |
Institution: | Unité de Glycobiologie Structurale et Fonctionnelle, UMR USTL/CNRS n 8576, batiment C9, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq, France. anne-sophie.vercoutter@univ-lille1.fr |
| |
Abstract: | Dextran derivatives are biosynthetic polyanionic polymers which exert some of the heparin properties such as regulating the activity of several heparin-binding growth factors. Based on a reproducible synthetic procedure, we have synthesized a new generation of dextran derivatives named dextran methylcarboxylate benzylamide sulfate (DMCBSu). Here we investigated the ability of a library of well-characterized DMCBSu to interact with platelet-derived growth factor-BB (PDGF-BB), which has essential roles during wound healing. Using gel mobility shift assay, our results indicate that benzylamide and sulfate groups act synergically to bind to PDGF-BB. Furthermore we show that depending on their chemical composition, functionalized dextrans are able to potentiate the mitogenic activity of PDGF-BB on human dermal fibroblasts. This enhancing effect is accompanied with changes in PDGF-BB-induced signaling events, as determined by the use of specific inhibitors and by western blot. Our results suggest that the use of such biopolymers combined with a local administration of the growth factor could increase the efficiency of the biomolecule activity in future therapeutic strategies. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|