异甘草素对小鼠脑缺血-再灌注损伤的保护作用

目的:研究异甘草素(ISL)对小鼠脑缺血再灌注损伤的保护作用。方法:采用反复阻断小鼠双侧颈总动脉造成脑缺血再灌注模型,断头法观察脑缺血再灌注小鼠耐缺氧能力;测定脑缺血再灌注小鼠全血粘度、红细胞压积和凝血时间;采用反相高效液相色谱法检测脑组织能量代谢水平。结果:ISL20,40mg·kg-1显著延长缺血再灌注小鼠断头后喘息时间(P<0.01),降低缺血再灌注小鼠全血粘度和红细胞压积(P<0.01),延长凝血时间(P<0.01);ISL(10～40mg·kg-1)剂量依赖性增加脑组织ATP含量、TAN水平和EC值,与模型组相比TAN水平和EC值分别增加了28%、42%、45%和41%、76%、90%,ATP含量依次恢复到假手术组的55%、75%、83%。结论:ISL可通过改善脑缺血再灌注小鼠异常血液流变学变化和提高脑组织能量代谢水平对脑缺血再灌注脑损伤起保护作用。

Protective Effects of Isoliquiritigenin against Cerebral Ischemia-reperfusion Injury in Mice

Objective: To investigate the protective effects of isoliquiritigenin (ISL) against cerebral ischemia-reperfusion injury in mice. Methods: The cerebral ischemia-reperfusion models in mice were made by repeated occlusion of bilateral common carotid arteries and reperfusion. To observe the hypoxia tolerance ability by means of recording the respiratory duration after cutting heads in cerebral ischemia-reperfusion mice. The whole blood viscosity, hematocrit and blood coagulation time were measured. The brain energy state was analyzed by reversed-phase high performance liquid chromatography. Results: ISL 20 and 40 mg·kg -1 intra-gastric infusion significantly prolonged the respiratory duration after head-cutting (P<0.01), decreased the whole blood viscosity and hematocrit, and prolonged the blood caogulation time (P<0.01). ISL (10～40 mg·kg -1) improved the brain energy metabolism in a dose-dependent manner. In cerebral ischemia-reperfusion mice treated with ISL (10～40 mg·kg -1 ), EC and TAN were enhanced by 28%,43%,45% and 41%,76%,90% compared with those in the model group, ATP levels were restored to 55%,75%,83% of sham value, respectively. Conclusion: The isoliquiritigenin has protective effects against cerebral ischemia-reperfusion injury in mice,which may be related to the improvement of abnormal hemorheological changes and the brain energy metabolism.