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Direct electron transfer between Alcaligenes eutrophus Z-1 hydrogenase and glassy carbon electrodes |
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| Authors: | Daniela D Schlereth Víctor M Fernández María Sánchez-Cruz Vladimir O Popov |
| Institution: | Instituto de Catálisis, CSIC, Campus Universidad Autónoma, Cantoblanco, 28049-Madrid Spain;Instituto de Quimica-Física “Rocasolano”, CSIC, Serrano 119, 28006-Madrid Spain;Institute of Biochemistry “A.N. Bach”, 33 Leninskiy Prospekt, 117.071-Moscow Russian Federation |
| Abstract: | In this work we report on the ability of the Alcaligenes eutrophus Z-1 hydrogenase immobilized on glassy carbon electrodes to support the electro-enzymatic reduction of NAD+ at ?700 mV vs. standard calomel electrode without external promoters or electron mediators.The electron-transfer process seems to be coupled to a relaxation of the quaternary structure of the enzyme molecules in contact with the electrode surface. |
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