复方蒲芩胶囊含量测定方法的改进 |
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引用本文: | 戚宝婵,孙苓苓,李子.复方蒲芩胶囊含量测定方法的改进[J].中国药品标准,2013,14(1):33-35. |
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作者姓名: | 戚宝婵 孙苓苓 李子 |
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作者单位: | 辽宁省食品药品检验所 |
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摘 要: | 目的:改进复方蒲芩胶囊的含量测定方法。方法:采用HPLC法同时测定复方蒲芩胶囊中黄芩苷和盐酸小檗碱的含量。色谱柱为Diamonsil C18(4.6 mm×200 mm,5μm),以乙腈-0.05 mol.L-1磷酸二氢钠溶液(29∶71)(用磷酸调节pH值至3.0)为流动相,检测波长为280 nm。结果:黄芩苷和盐酸小檗碱的线性范围分别为0.056 57~0.848 6μg(r=0.999 9)和0.254 9~3.823 5μg(r=0.999 9);平均回收率分别为98.0%(RSD=2.2%),97.9%(RSD=0.97%)。结论:方法简便、准确,重复性好,可用于复方蒲芩胶囊质量控制。
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关 键 词: | 复方蒲芩胶囊 黄芩苷 盐酸小檗碱 高效液相色谱法 |
收稿时间: | 2012/2/13 0:00:00 |
Improvement of Determination of Fufang Puqin Capsules |
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Authors: | Qi Baochan Sun Lingling and Li Zi |
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Institution: | (Liaoning Institute for Food and Drug Control,Shenyang 110023) |
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Abstract: | Objective: To improve the method for determination of Fufang Puqin Capsules. Methods: To establish an HPLC method for simultaneous determination of baicalin and berberine hydroehloride in Fufang Pulqin Capsules. The chromatographic separation was achieved on a Diamonsil CIs (4. 6 mm × 200 mm, 5 μm) column with acetonitrile-0.05 mol · L -1 sodium dehydrogenate phosphate(29 :71 ) (adjusted to pH 3.0 with phosphoric acid)as mobile phase and the detective wavelength was set at 280 nm. Results: The calibration carves of baicalin and berberine hydrochloride were linear in the ranges of 0. 056 57 - 0. 848 6 μg ( r = 0.999 9 ) , 0. 254 9 - 3. 823 5·g (r =0. 999 9) respectively. The average recoveries were 98. 0% with RSD 2. 2% , 97. 9% with RSD 0. 97% respectively. Conclusion: The method is simple, accurate and reproducible for quality control of Fufang Pulqin capsules. |
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Keywords: | Fufang Pulqin capsule baicalin berberine hydrochloride HPLC |
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