| RP-HPLC法测定绞股蓝中绞股蓝皂苷B的含量 |
| |
| 引用本文： | 刘春祥,张群,谢二磊,周国平. RP-HPLC法测定绞股蓝中绞股蓝皂苷B的含量[J]. 中国药品标准, 2013, 14(1): 7-9 |
| |
| 作者姓名： | 刘春祥 张群 谢二磊 周国平 |
| |
| 作者单位： | 江西省药品认证中心;江西省食品药品检验所 |
| |
| 摘 要： | 目的：采用反相高效液相色谱法测定绞股蓝中绞股蓝皂苷B的含量。方法：采用Agilent Eclipse XDB-C18色谱柱（4．6mm×250mm，5μm），以乙腈-0．5％磷酸（38：62）为流动相，流量1．0mL·min-1，检测波长210nm，柱温30℃。结果：绞股蓝皂苷B进样量在0．0402～2．012μg范围内与峰面积呈良好线性关系，平均回收率为99．52％，RSD为1．69％（n=6）。结论：该方法简便、准确，重复性好，可作为绞股蓝的质量控制方法。
|
| 关 键 词： | 绞股蓝 绞股蓝皂苷B 反相高效液相色谱法 |
| 收稿时间： | 2012-03-16 |
RP-HPLC Determination of Gypenoside B in Fiveleaf Gynostemma Herb (Gynostemma Pentaphylli Herba) |
| |
| Liu Chunxiang,Zhang Qun,Xie Erlei and Zhou Guoping. RP-HPLC Determination of Gypenoside B in Fiveleaf Gynostemma Herb (Gynostemma Pentaphylli Herba)[J]. , 2013, 14(1): 7-9 |
| |
| Authors: | Liu Chunxiang Zhang Qun Xie Erlei Zhou Guoping |
| |
| Affiliation: | 1.Jiangxi Provicial Certification Committee for Drugs,Nanchang 330046;2.Jiangxi Institute for Food and Drug Control,Nanchang 330046) |
| |
| Abstract: | Objective： To develop an RP-HPLC method for the determination of gypenoside B in Fiveleaf Gynostemma Herb. Methods： The separation was performed at 30 ℃on a Agilent Eclipse XDB-Clscolumn （4. 6 mm× 250 mm, 5 μm）with a mobile phase of acetonitrile-0. 5% phosphoric acid（38： 62）. The flow rate was 1.0 mL · min-1 , and the detecting wavelength was set at 210 nm. Results： The linear range of Gypenoside B was from 0. 040 2-2. 012 μg. The average recovery of Gypenoside B was 99. 52% ,RSD was 1.69% （n =6）. Conclusion： The method is simple and accurate with good reproducibility. It can be used for the quality control of Fiveleaf Gynostemma Herb. |
| |
| Keywords: | Fiveleaf Gynostemma Herb Gypenoside B RP-HPLC |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《中国药品标准》浏览原始摘要信息 |
|
点击此处可从《中国药品标准》下载全文 |
