| 银杏叶提取物对糖尿病大鼠视网膜神经节细胞Bax和Bcl-2基因表达的影响 |
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| 引用本文： | 项楠,林晗,李贵刚,刘恒明. 银杏叶提取物对糖尿病大鼠视网膜神经节细胞Bax和Bcl-2基因表达的影响[J]. 华中科技大学学报(医学版), 2008, 37(2): 243-247. DOI: 10.3870/j.issn.1672-0741.2008.02.028 |
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| 作者姓名： | 项楠 林晗 李贵刚 刘恒明 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院眼科,武汉,430030 |
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| 摘 要： | 目的 观察银杏叶提取物(extract of ginkgo biloba leaf, EGB)对糖尿病大鼠视网膜神经节细胞凋亡相关基因Bax和Bcl-2表达的影响,探讨EGB防治糖尿病性视网膜病变的作用机制.方法 选择健康成年Wistar大鼠30 只, 随机分成正常对照组、模型组和EGB治疗组.腹腔内注射链脲佐菌素(STZ) 诱发大鼠糖尿病.EGB治疗组给予EGB溶液灌胃12周,模型组和对照组灌服等体积生理盐水,随后制备视网膜石蜡切片,行免疫组化染色, 并对染色结果行计算机图像分析.透射电镜观察各组神经节细胞的超微结构变化.结果 Bax在模型组神经节细胞的表达较正常对照组显著增强,在EGB治疗组的表达强度较模型组减弱;Bcl-2在EGB治疗组表达较模型组明显增强,差异均有统计学意义(P<0.01).EGB治疗组神经节细胞超微结构的损害明显减轻.结论 EGB可减轻糖尿病大鼠视网膜神经节细胞的超微结构损伤,减轻细胞凋亡的发生,其机制可能与EGB调节凋亡相关基因Bax和Bcl-2的表达有关.
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| 关 键 词： | 银杏叶提取物 糖尿病视网膜病变 视网膜神经节细胞 Bax Bcl-2 细胞凋亡 银杏叶提取物 糖尿病大鼠 视网膜神经节细胞 基因表达 影响 Diabetic Mellitus Rats Cells Retinal Genes Expression Ginkgo biloba Leaf Extract 调节 发生 细胞凋亡 微结构损伤 损害 细胞超微结构 统计学意义 |
| 修稿时间： | 2007-11-19 |
Effect of Extract of Ginkgo biloba Leaf on the Expression of Bax and Bcl-2 Genes in Retinal Ganglial Cells of Rats with Diabetic Mellitus |
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| Xiang Nan,Lin Han,Li Guigang et al. Effect of Extract of Ginkgo biloba Leaf on the Expression of Bax and Bcl-2 Genes in Retinal Ganglial Cells of Rats with Diabetic Mellitus[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2008, 37(2): 243-247. DOI: 10.3870/j.issn.1672-0741.2008.02.028 |
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| Authors: | Xiang Nan Lin Han Li Guigang et al |
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| Affiliation: | Xiang Nan,Lin Han,Li Guigang et al Department of Ophthalmology,Tongji Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430030 |
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| Abstract: | Objective To investigate the effect of extract of Ginkgo biloba leaf(EGB) on the expression of apoptosis-related genes Bax and Bcl-2 in retinal ganglial cells of rats with diabetic mellitus,and discuss the mechanism of EGB preventing and curing diabetic retinopathy.Methods Thirty healthy adult Wistar rats were chosen and divided into control group,model group,and EGB-treated group randomly.Diabetic mellitus was induced by intraperitoneal injection of streptozotocin(STZ).In EGB-treated group,the rats were la... |
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| Keywords: | extract of Ginkgo biloba leaf diabetic retinopathy retinal ganglial cells Bax Bcl-2 apoptosis |
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