实验性自身免疫性脑脊髓炎大鼠Th1、Th2和Th17免疫反应检测

目的探讨不同类型免疫反应在实验性自身免疫性脑脊髓炎(EAE)发病中的作用。方法 24只SD大鼠随机均分为EAE组和对照组,通过行为观测和大脑微观形态学确认免疫诱导的EAE模型,用ELISA法检测淋巴结和脾细胞培养上清液中IL-4、IFN-γ和血清中IgG水平,流式细胞术检测淋巴结和脾细胞中IL-17及Foxp3细胞频数。结果与对照组相比,EAE组IgG、IFN-γ、IL-17及IL-4水平均明显增高(P<0.05或P<0.01)。结论 Th1、Th2和Th17免疫细胞在EAE的发病均起着重要的作用。

Detection of Th1,Th2 and Th17 immune responses in rats with experimental autoimmune encephalomyelitis

Objective To investigate the effects of different types of immune reactions on the pathogenesis of experimental autoimmune encephalomyelitis(EAE). Methods Twenty-four SD rats were equally randomized into EAE group and control group.The immune-induced EAE model was confirmed by praxiology and microscope morphology. The levels of IL-4 and IFN-γ in the supernatant of cultured lymphocytes and splenocyte and the level of IgG in serum were detected by ELISA.The cell frequencies of IL-17 and Foxp3 in the lymphocytes and splenocyte were measured by flow cytometry. Results The levels of IgG,IFN-γ,IL-17 and IL-4 in EAE group were all significantly higher than those in control group(P<0.05 or P<0.01). Conclusion The Th1,Th2 and Th17 immune reactions play an important role in the pathogenesis of EAE.