高同型半胱氨酸血症对慢性应激模型大鼠行为学及神经生化的影响

目的研究高同型半胱氨酸血症(Hhcy)对慢性不可预见应激模型大鼠行为学、神经递质及神经营养因子水平的影响。方法以高蛋氨酸饮食喂养大鼠制作Hhcy模型(Hhcy组,12只);另选24只大鼠,均分为普食组和对照组,均给予普通饮食喂养;Hhcy组与普食组给予慢性不可预见性刺激。比较三组的学习记忆能力、海马组织5-羟色胺(5-HT)和5-羟基吲哚乙酸(5-HIAA)含量以及脑脊液中神经营养因子(BDNF)水平。结果水迷宫实验中,Hhcy组目的象限停留时间比普食组短(P<0.05);Hhcy组海马5-HIAA含量和脑脊液中BDNF含量均较普食组低(P<0.05)。结论Hhcy可致慢性应激模型大鼠学习记忆能力的下降,加重神经递质及生长因子水平的异常,可能为抑郁症的危险因素之一。

Influence of hyperhomocysteinemia on behavioral and neurochemical alterations in a chronic mild unconditioned stress rat model

Objective To study the influence of hyperhomocysteinemia on behavior,neurotransmiters and neurotrophic factor in rats. Methods The hyperhomocysteinemia animal model was established by hyper-methionine diet in 12 rats(group A).Another 24 rats fed with conventional diet were divided into groups of B and C with 12 rats each.A chronic mild unconditioned stress was performed in groups of A and B.The cognitive ability,contents of 5-hydroxytryptamine(5-HT) and 5-hydroxyindoleacetic acid(5-HIAA) in hippocampus tissues and brain-derived nutrition factor(BDNF) in cerebral spinal fluid(CSF) were compared among three groups. Results In morris water maze test,the time spent in target quadrant was less in group A than that in group B(P<0.05).The levels of 5-HIAA in the hippocampus and BDNF in CSF were lower in group A than those in group B(P<0.05). Conclusion Hyperhomocysteinemia can reduce the cognitive ability,deteriorate the abnomality of monoamine transmitters and BDNF in rats,which may be one of the risk factors for depression.