| AB-8大孔树脂分离纯化苜蓿总皂苷的研究 |
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| 引用本文： | 余从洪,郭江宁,温锦涛,沈惠宾.AB-8大孔树脂分离纯化苜蓿总皂苷的研究[J].中外健康文摘,2006(2). |
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| 作者姓名： | 余从洪 郭江宁 温锦涛 沈惠宾 |
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| 作者单位： | 广东省中山市中健药物研究所有限公司 528437 |
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| 摘 要： | 目的研究大孔树脂提取苜蓿总皂苷的工艺。方法以苜蓿总皂苷的洗脱率和纯化度为指标,用不同浓度的乙醇进行洗脱,对AB-8大孔树脂吸附剂提取苜蓿总皂苷的工艺进行了筛选。结果最佳工艺为药液上样量为3BV(树脂床体积),4BV蒸馏水洗去水溶性杂质,4BV的70%乙醇洗脱。结论经AB-8大孔树脂处理后的苜蓿总皂苷可达30%以上,而且工艺简便,分离效果好,能满足大生产的要求。
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| 关 键 词： | 大孔树脂 苜蓿总皂苷 纯化 |
Study on purification process of total saponin of alfalfa with AB-8 macroreticular resin |
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| YU Cong-hong,GUO Jiang-ning,WEN Jing-tao,SHEN Hui-bin.Study on purification process of total saponin of alfalfa with AB-8 macroreticular resin[J].World Health Digest Medical Monthly,2006(2). |
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| Authors: | YU Cong-hong GUO Jiang-ning WEN Jing-tao SHEN Hui-bin |
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| Institution: | ZhongShan Zhongjian Institute of materia Medica Co.Ltd. Zhongshan GuangDong 528437 |
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| Abstract: | AIM To study the extraction process of total saponin of alfalfa with macroreticular resin. METHODS The extraction process of total saponin of alfalfa with AB-8 macroporous resin adsorbent was selected with different concentration of ethanol for gradient elution by taking the elusive and purified ratio of total saponin as reference. RESULTS The AB-8 macroporous resin had the best separating efficientcy when the volume of drug was 3BV(resin bed volume),washed with 4BV of distilled water and eluted with 4BV of 70% ethanol. CONCLUSION The extraction process is simple and convenient with good effect of separation, which can meet industrial requirements. |
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| Keywords: | macroreticular resin total saponin of alfalfa purification |
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