STZ诱导裸鼠糖尿病模型的建立及观察

目的 通过链脲佐菌素(STZ)腹腔注射制备裸鼠糖尿病模型,观察其血糖及胰腺病理学改变.方法 选取裸鼠80只,随机分成为3个实验组和1个对照组,实验组分别行一次性腹腔注射100 mg/Kg、150 mg/Kg、200 mg/Kg剂量的STZ,对照组注射柠檬酸缓冲液.利用便携式血糖仪监测血糖值,观察糖尿病裸鼠模型的造模情况及其胰腺病理学改变.结果 注射剂量为150 mg/Kg STZ组,给药1周后测实验裸鼠血糖,该组有17只裸鼠达到成模标准,在10周时检测均并未出现转阴者.糖尿病裸鼠胰腺组织光镜下(HE染色)观察发现胰岛细胞减少,胰岛细胞出现变性、坏死.模型鼠糖尿病症状明显.结论 通过腹腔一次性注射150 mg/Kg剂量的STZ可有效地建立起糖尿病裸鼠模型,适用于大批模型的建立及组织工程相关研究.

Establishment and Observation of Nude Mouse Diabetes Mellitus Model Induced by Streptozotocin

Objective Establish a diabetic model in nude mice by intraperitoneal injection of streptozotocin and observe changes of blood glucose and pancreatic tissue.Methods Eighty nude mice were divided into four groups separately intraperitoneal injected by 0 mg/Kg,100 mg/Kg,150 mg/Kg,200 mg/Kg STZ.To evaluate the model establishment,the blood glucose of diabetic nude mice was detected with a portable glucose meter.Pancreatic pathological changes were observed under light microscope.Results In the group injected by 150 mg/Kg STZ,17 mice were found with high blood glucose after 1 week and kept high blood glucose over 16.7 mmol/L over 10 weeks.The evidences from optical microscope observation further confirmed pathological changes on pancreatic islets cells of diabetic models along with the diabetic symptoms(polydipsia,polyphagia,diuresis).Conclusion With the dose of 150 mg/Kg of STZ through once intraperitoneal injection,we can effectively induce nude mice models of diabetes mellitus,especially in a large number models and the study of tissue engineering.