金雀异黄素对氧化损伤的人晶状体上皮细胞凋亡的防护作用 |
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引用本文: | 林瑶,黄秀榕,祁明信,黄露芬,郭娜,唐宋文,陈旭征.金雀异黄素对氧化损伤的人晶状体上皮细胞凋亡的防护作用[J].江西中医学院学报,2014,0(1):67-70. |
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作者姓名: | 林瑶 黄秀榕 祁明信 黄露芬 郭娜 唐宋文 陈旭征 |
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作者单位: | 林瑶(福建中医药大学中西医结合学院 福建福州350108);黄秀榕(福建中医药大学中西医结合学院 福建福州350108); 祁明信 (福建中医药大学附属第二人民医院眼科 福建福州350003); 黄露芬(福建中医药大学中西医结合学院 福建福州350108); 郭娜 (福建中医药大学附属第二人民医院眼科 福建福州350003); 唐宋文(福建中医药大学中西医结合学院 福建福州350108);陈旭征(福建中医药大学中西医结合研究院 福建福州350108); |
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基金项目: | 福建省教育厅基金(JA12177) |
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摘 要: | 目的:探讨金雀异黄素(Gen)对氧化损伤的人晶状体上皮细胞(HLEC)凋亡有无抑制作用。方法:本研究在终浓度3×10^-4mol/L H2O2培养液中复制HLEC凋亡模型,并以雌二醇(E2)作为阳性对照,采用流式细胞仪(FCM)检测HLEC凋亡率和线粒体膜电位(ΔΨM)的变化。结果:FCM结果显示:H2O2组HLEC凋亡率显著高于空白对照组;E2和Gen组凋亡率均低于H2O2组(P〈0.01)。H2O2组线粒体膜电位比对照组显著下降;E2组和Gen组的线粒体膜电位均比H2O2组升高(P〈0.01)。结论:Gen可减轻实验性氧化损伤的HLEC凋亡程度。
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关 键 词: | 金雀异黄素 氧化损伤 晶状体上皮细胞 凋亡 |
Genistein Protecting Against Apoptosis of Human Lens Epithelial Cell Injured by Oxidation |
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Institution: | LIN Yao, HUANG Xiu- rong, QI Ming -xin, HUANG Lu- feng, GUO Na, TANG Song -wen, CHEN Xu - zheng( 1. College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China; 2. Department of Ophthalmology, the Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, China; 3. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China) |
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Abstract: | Objective: To investigate if Genistein( Gen) inhibits the apoptosis of human lens epithelial cell( HLEC) resulted from the experimental oxidative injury. Methods: HLECs were incubated with 3 × 10^- 4mol / L H2O2. And make estradiol( E2) as a positive control. HLECs apoptosis and apoptosis rate were measured by FCM. Results: Apoptosis rate in H2O2group was significandy higher than that in control group,E2and Gen group. The mitochondria transmembrane potential in H2O2group reduced obviously,comparing to control group,E2and Gen group. Conclusion: Gen may decrease the apoptosis of HLEC resulted from the experimental oxidative injury,providing scientific evidence for pursue effective medicine to prevent and treat cataract. |
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Keywords: | Genistein Oxidative damage Human lens epithelial cell Apoptosis |
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