红腺忍冬叶抗氧化有效部位多糖的含量变化研究

目的：建立红腺忍冬叶抗氧化有效部位中多糖的含量测定方法，并对不同批次红腺忍冬叶抗氧化有效部位中的多糖含量进行测定及比较。方法：采用苯酚-硫酸法测定总糖含量，3，5-二硝基水杨酸法测定还原糖的含量，取两者差值为多糖含量，以测定红腺忍冬叶抗氧化有效部位中的多糖含量。结果：15批浙江产红腺忍冬叶水提液中多糖含量为5．045～12．348 mg/mL，水提液30％乙醇沉淀后滤液中多糖含量为4．880～11．944mg/mL，水提液30％乙醇沉淀物中多糖含量为0．015～0．119 mg/mL。结论：建立的多糖测定方法简单、准确、适用性良好，可用于红腺忍冬叶抗氧化有效部位中多糖含量的测定。

Changes of Polysaccharide Content in Antioxidant Effective Fraction of Leaves of Lonicera hypoglauca Miq

Objective：To establish a method for the quantitative determination of polysaccharide in antioxidant effective fraction of the Leaves of Lonicera hypoglauca Miq, and to study the comparison of the content of polysaccharide in different batches with the method . Methods：The content of total sugar was determined by phenol -sulfuric acid method, while reducing sugar was determined by 3,5-dinitrosalicylic acid method .The content of polysaccharide was the differential value between total sugar and reducing sugar by detecting it in antioxidant effective fraction of the leaves of Lonicera hypoglauca Miq.Results： The contents of polysaccharide of leaves of Lonicera hypoglauca Miq.in 15 batches from Zhejiang province were 5.045-12.348mg/mL in water extraction, 4.880-11.944mg/mL in the filtrate of 30%ethanol precipitating action , 0.015-0.119mg/mL in the precipitation of 30%ethanol precipitating action . Conclusions：The method is simple , accurate and suitable for the determination and comparison of the contents of polysaccharide in antioxidant effective fraction of the Leaves of Lonicera hypoglauca Miq.