蒺藜总皂苷含量测定研究 |
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引用本文: | 李艳,胡蓉,王丽静,漆小勇,曾宪仪. 蒺藜总皂苷含量测定研究[J]. 江西中医学院学报, 2014, 0(1): 59-61 |
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作者姓名: | 李艳 胡蓉 王丽静 漆小勇 曾宪仪 |
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作者单位: | 李艳 (江西省药物研究所 江西南昌330029); 胡蓉 (江西省药物研究所 江西南昌330029); 王丽静 (江西省药物研究所 江西南昌330029); 漆小勇(江西远健药业有限公司 江西南昌330100); 曾宪仪 (江西省药物研究所 江西南昌330029); |
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摘 要: | 目的:研究蒺藜总皂苷的含量测定方法。方法:以26-O-β-D-吡喃葡萄糖-(25R)-5α-呋甾烷-20(22)-烯-2α,3β,26-三醇-3-O-β-D-吡喃葡萄糖-(1→4)-β-D-吡喃半乳糖苷为对照品(简称化合物Ⅱ对照品),建立紫外可见分光光度法测定蒺藜总皂苷的含量,显色试剂为E试剂和硫酸乙醇溶液,测定波长为520nm。结果:在0.06-0.2mg的范围内对照品量与吸收值之间有良好的线性关系,回归方程为Y=3.48X-0.13,相关系数r=0.9992(n=5),平均回收率为97.28%,RSD为1.5%(n=5)。结论:以蒺藜中单体成分化合物Ⅱ为对照品进行含量测定,方法可控,重现性好,结果准确,可用于本品质量控制。
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关 键 词: | 蒺藜 总皂苷 紫外可见分光光度法 |
Study on Determination of Total Saponins Content of Tribulus TerrestrisL. |
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Affiliation: | LI Yan, HU Rong, WANG Li -jing, QI Xiao- yong, ZENG Xian- yi( 1 Jiangxi Institute of Materia Medica, Nanchang 330029, China ;2 Jiangxi Yuanjian Pharmaceutical Industry ltd. , Nanchang 330100, China) |
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Abstract: | Objective: To establish the method for total saponins content determination of Tribulus terrestris L. crude extract. Methods: The total saponins was colored with chromogenic agent E reagent and sulfuric acid ethanol solution and determined by UV spectrophotometry at 520nm with a standard substance named 26- O- β- D- glucopyranosyl-( 25R)- 5α- furostan- 20( 22)- en- 2α, 3β,26- triol- 3- O- β- D- glucopyranosyl-( 1→4)- β- D- galactopyranoside( referred to as the compound Ⅱ control). Results: Diosgenin had good linear relationship in 0. 06 - 0. 2mg,the regression equation was Y = 3. 48X- 0. 13,r = 0. 9992,average recovery was 97. 28%,RSD = 1. 5%( n = 5). Conclusion: The method is controllable,reproducible,with a high accurate rate and can be used to control quality. |
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Keywords: | Total Saponins of Tribulus Terrestris the Compound Ⅱ UV Spectrophotometry |
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