| 用逆转录─多聚酶链反应检测卵巢癌及大肠癌患者多药耐药基因的表达 |
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| 引用本文： | 徐建业,周琦,沈平,李福沛,李伟道.用逆转录─多聚酶链反应检测卵巢癌及大肠癌患者多药耐药基因的表达[J].重庆医学,1997(1). |
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| 作者姓名： | 徐建业 周琦 沈平 李福沛 李伟道 |
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| 作者单位： | 重庆肿瘤研究所!630030 |
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| 摘 要： | 应用逆转录─多聚酶键反应（RT－PCR）检测卵巢癌12例，大肠癌15例，肝癌2例肿瘤多药耐药基因（MDR1）表达。以长春新碱（VCR）敏感细胞株KB和耐药株KBr分别作阴性及阳性对照，卵巢癌患者MDR1表达增高者为50％（6／12），大肠癌增高者75％（10／15）卵巢癌MDR1表达低者化疗效果较好；大肠癌患者MDR1表达高者似与术前局部用化疗药有关。
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| 关 键 词： | 肿瘤多药耐药基因 逆转录多聚酶链反应 卵巢癌 大肠癌 |
DETERNIRATION OF MIDR_1 GENE EXPRESSION IN PATIENTS WITH OVARIAN CANCER OF COLORECTAL CANCER WITH RT-PCR |
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| Xu Jianye, et al.DETERNIRATION OF MIDR_1 GENE EXPRESSION IN PATIENTS WITH OVARIAN CANCER OF COLORECTAL CANCER WITH RT-PCR[J].Chongqing Medical Journal,1997(1). |
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| Authors: | Xu Jianye |
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| Abstract: | Used VCR-sensitive KB cell line and VCR-resistant KBr cell line as negative and positive control respectively,the authors deterned Multiple Drug Resistant qene 1 (MDR1) mRNA in patients with ovarian cancer or colorectal cancer with RT-PCR. High level expression of MDR1 was found in 6/12 of ovarian cancer and 10/15 of colorectal cancer. The deternination of MDR1 may be helpfulin designing chemotherapeutic protocols in the patients with luqh level expression of MDR1 |
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| Keywords: | MDR_1 RT-PCR Ovarian cancer coloretalcancer |
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