香加皮杠柳苷联用TRAIL对胃癌SGC-7901和MGC-803细胞的作用及其机制

目的:探讨香加皮杠柳苷(cortex periplocae,CPP)联用肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor related apoptosis inducing ligand,TRAIL)对胃癌细胞的作用及其机制.方法:人胃癌细胞系SGC-7901、MGC-803培养完成后,采用50、100、200 ng/ml的CPP和1μg/ml的TRAIL单用或联合处理.MTS法检测SGC-7901和MGC-803细胞的增殖情况,流式细胞术检测其凋亡率,Westcm boltting检测pro-BID、Mcl-l、cleaved caspase-3、DR4、DR5的表达水平.结果:SGC-7901和MGC-803细胞经CPP (50、100、200 ng/ml)和TRAIL(1 μg/ml)联合处理24 h后,细胞增殖率明显低于空白对照组和对应的CPP各剂量单独处理组(/＜0.05或P＜0.01).SGC-7901和MGC-803细胞凋亡率均明显高于空白对照组和对应的CPP各剂量单独处理组(P＜0.05或P＜0.01).SGC-7901和MGC-803细胞中pro-BID、Mcl-1表达水平明显降低(均P＜0.05),cleaved caspase-3表达水平明显升高(P＜0.05),DR4和DR5受体的表达水平升高(均P＜0.05).结论:CPP协同TRAIL可明显诱导人胃癌SGC-7901和MGC-803细胞凋亡,增强人胃癌细胞对TRAIL的敏感性.

Effect of combined treatment of cortex periplocae and TRAIL on gastric cancer SGC-7901 and MGC-803 cells and its mechanism

Objective:To investigate the effect of combined treatment of cortex periplocin (CPP) and tumor necro sis factor related apoptosis inducing ligand (TRAIL) on gastric cancer cells and to explore the mechanism.Methods:After routine culture,the gastric cancer cell lines (SGC-7901 and MGC-803) were treated with CPP (at concentrations of 50,100,200 ng/ml) or TRAIL (1 μg/ml) or in combination of these two.The cell proliferation was detected by MTS,the apoptosis was detected by Flow cytometry,and the expression levels of pro-BID,Mcl-1,cleaved caspase-3,DR4 and DR5 were detected by Western blotting.Results:Compared with the control group and each CPP single treatment group,MTS assay demonstrated that CPP (50,100,200 ng/ml) in combination with TRAIL (1 μtg/ml) significantly inhibited the proliferation of gastric cancer SGC-7901 and MGC-803 cell lines (all P＜0.05 or P＜0.01).Flow cytometry demonstrated that the apoptosis rate of gastric cancer cells in combined treatment group was significantly higher than that of control group or each CPP single treatment group (P＜0.05 or P＜0.01).Western blotting demonstrated that combined treatment for 24 h significantly decreased the expression of pro-BID and Mcl-1 (P＜0.05),but increased the expression levels of cleaved caspase-3,DR4 and DR5(P＜0.05).Conclusion:CPP in combination with TRAIL could significantly induce the apoptosis of gastric cancer SGC-7901 and MGC-803 cell lines and increase the susceptibility of cancer cells to TRAIL.