磷酸化PKCγ在正常小鼠脑内的免疫组织化学定位

为了研究磷酸化的蛋白激酶Cγ(pPKCγ)在小鼠脑内的区域分布,为进一步了解其在脑内的功能提供线索。我们将BALB/c小鼠脱臼处死,灌流取脑,行免疫组织化学染色,观察pPKCγ阳性反应产物在脑内的分布。观察到pPKCγ阳性产物主要见于神经元的胞浆和突起中,在正常小鼠脑内表达广泛。仅很少量胶质细胞被染色,着色浅淡。海马CA1区锥体细胞,小脑的浦肯野氏细胞,大脑新皮质V层细胞,边缘前皮质、岛皮质的深层细胞以及前梨皮质的颗粒层细胞,外侧嗅束核、杏仁基底外侧核和杏仁外侧核的神经元,以及穹窿、锥体束纤维等均有强或较强的pPKCγ表达;吻侧丘脑大多数核团,尾侧丘脑的内侧膝状体核及外侧膝状体的背侧核,脑干耳蜗背侧核的表浅部位,三叉神经感觉核簇及巨细胞网状结构神经元中均有弱阳性表达。本实验研究揭示正常状态下小鼠脑内pPKCγ表达广泛,但有区域特异性,提示PKCγ可能参与正常状态下脑内部分脑区/核团神经元的功能活动。

THE IMMUNOHISTOCHEMICAL LOCALIZATION OF THE PHOSPHORYLATED PROTEIN KINASE C GAMMA IN THE NORMAL MOUSE BRAIN

In order to provide morphological basis for further functional study,the topographical distribution of the phosphorylated protein kinase C gamma subtype(pPKCγ) in the brain were observed.BALB/c mice were sacrificed with cervical dislocation and fixed with perfusion.The brains were sectioned and immunohistochemically stained for pPKCγ.Phosphorylated PKCγ-like immunoreactivities were widely but region-specifically distributed in the mouse brain.The positive products were mainly seen in neurons,at both perikaria and processes.Few glial cells were faintly stained.The pyramidal neurons in CA1 of hippocampus,Purkinje cells in cerebellum,the neurons in layer V of neocortex,the deep layers of prelimbic and insular cortex,granular layer of prepiriform cortex,basolateral amygdaloid nucleus,lateral amygdaloid nucleus,as well as fornix and pyramidal tract were all showed strong immunoreactivity for pPKCγ.In addition,neurons in most nuclei of rostral thalamus,medial and dorsolateral geniculate nuclei,superficial area of dorsal cochlear nucleus,nucleus of spinal trigeminal tract and gigantocellular reticular nucleus were weakly stained.The results provide data on the topographic distribution of pPKCγ in mouse brain and indicate that PKCγ might involve the functional activity of neurons at related areas in normal condition.