| 大黄素对体外培养Cajal间质细胞钙离子振荡功能的影响 |
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| 引用本文： | 李春穴,童卫东,刘宝华,龙在云. 大黄素对体外培养Cajal间质细胞钙离子振荡功能的影响[J]. 结直肠肛门外科, 2006, 12(1): 2-5 |
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| 作者姓名： | 李春穴 童卫东 刘宝华 龙在云 |
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| 作者单位： | 重庆第三军医大学大坪医院普外科,400042 |
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| 摘 要： | 目的:探讨大黄素对体外培养Cajal间质细胞钙离子振荡的影响,从而进一步揭示大黄素等促进胃肠动力的分子机制方法:体外培养小鼠ICC,体外添加大黄素,应用钙离子指示剂标记细胞内钙离子含量,在激光共聚焦显微镜下动态观察其钙离子振荡功能的变化。结果:免疫荧光鉴定结果证实体外培养细胞为ICC,体外培养ICC具有钙离子振荡功能,大黄素能显著增强其钙离子振荡的频率和振幅。结论:大黄素能显著增强体外培养ICC钙离子振荡功能,可能是其促进胃肠动力的重要机制。
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| 关 键 词： | 大黄素 Cajal间质细胞 钙离子振荡 |
| 收稿时间： | 2006-02-15 |
| 修稿时间： | 2006-02-15 |
Effects of emodin on the function of Ca2+ oscillation of ICC cultured in vitro |
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| Li Chunxue, Tong Weidong, Liu Baohua, et al. Effects of emodin on the function of Ca2+ oscillation of ICC cultured in vitro[J]. Journal of Colorectal & Anal Surgery, 2006, 12(1): 2-5 |
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| Authors: | Li Chunxue Tong Weidong Liu Baohua et al |
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| Abstract: | Objective:To study the effects of emodin on the function of Ca~ 2 oscillation in interstitial cells of Cajal (ICC) cultured in vitro.Methods:ICC of murine small intestine were cultured in vitro. The function of Ca~ 2 oscillation associated slow waves before and after adding emodin was detected under confocal microscope. Results:Emodin could significantly increase the frequency (about 34 circles / min) and amplitude of vibration.Conclusion:ICC of murine small intestine cultured in vitro show morphologic characters. Cultured ICC of murine small intestine in vitro have function of Ca~ 2 oscillation linked slow waves and emodin can significantly increase its frequency and amplitude of vibration. |
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| Keywords: | Emodin Interstitial cells of Cajal(ICC) Ca~2 oscillation |
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