交感性眼炎眼组织中共刺激分子表达的变化

目的:探讨交感性眼炎眼组织(虹膜睫状体、脉络膜、视网膜)中共刺激分子B7-1,B7-2,CD28和CTLA-4的表达意义。方法:交感性眼炎眼球石蜡标本4例,行常规石蜡切片,免疫组织化学方法探讨交感性眼炎各部分眼组织中上述分子的表达,并与正常人眼组织中各分子的表达进行比较。结果:正常人眼组织虹膜睫状体中有B7-2分子,无B7-1,CTLA-4和CD28分子;脉络膜中有B7-1分子,但无B7-2,CTLA-4和CD28分子;视网膜内无B7-1,B7-2,CTLA-4和CD28分子。而交感性眼炎的虹膜睫状体和脉络膜中均有B7-1,B7-2,CTLA-4和CD28分子,视网膜的色素上皮细胞有B7-1和B7-2分子表达,而无CD28和CTLA-4分子表达,视网膜内可见少量B7-1,B7-2,CTLA-4和CD28分子表达。结论:眼组织中B7与CD28和CTLA-4分子之间的相互作用与交感性眼炎患者葡萄膜处于持续免疫激活状态密切相关。

Immunohistochemical study of costimulatory molecules in sympathetic ophthalmia

AIM:To examine the specimens of iris,ciliary body and retina from patients with sympathetic ophthalmia for the expression of costimulatory molecule CD28,CTLA-4 and its ligands B7-1(CD80)and B7-2(CD86).METHODS:The paraffin-embedded sections of sympathetic ophthalmia were obtained based on the specimens of 4 patients with sympathetic ophthalmia and 2 healthy persons,and analyzed by immunohistochemical method with polyclonal antibody probes for B7-1,B7-2,CD28 and CTLA-4 molecules.RESULTS:The specimens of normal iris and ciliary body showed B7-2 expression.B7-1 was found in the normal choroids.B7,CD28 and CTLA-4 molecules did not express in the normal retina.However,in the sympathetic ophthalmia,the iris and ciliary body showed B7-1,B7-2,CD28 and CTLA-4 molecules expression.Approximately 100% of retina pigment epithelium cells expressed B7-1 and B7-2 molecules,but not CD28 or CTLA-4 molecules.In the specimens of retina,there was low expression of B7-1,B7-2,CD28 and CTLA-4 molecules.CONCLUSION:The results of this study indicate that the interaction between B7 and CD28,CTLA-4 may contribute to the sustained immune activation in the uvea of the patients with sympathetic ophthalmia.