| 抗间日疟原虫红内期单克隆抗体的研制和鉴定 |
| |
| 引用本文： | 罗树宏,刘多,叶炳辉,舒衡平,付冉定.抗间日疟原虫红内期单克隆抗体的研制和鉴定[J].中南大学学报(医学版),1995(3). |
| |
| 作者姓名： | 罗树宏 刘多 叶炳辉 舒衡平 付冉定 |
| |
| 作者单位： | 南京医科大学寄生虫学教研室 |
| |
| 摘 要： | 用Ｐｅｒｃｏｌｌ密度梯度离心法从志愿者血液中分离出感染间日疟原虫的红细胞。以此作为免疫原，免疫Ｂａｌｂ／ｄ小鼠，获得５株杂交瘤细胞系。融合率平均为４５％，抗体阳性率为０．６６％。用ＩＦＡｆｏＩＰＡ染色进行免疫组化分析，４Ｂ２和８Ｅ３分泌的单克隆抗体呈较强的阳性反应，与约氏疟原虫红内期无交叉反应。
|
| 关 键 词： | 抗体类，单克隆 间日疟原虫 杂交瘤 免疫酶技术 小鼠 |
PRODUCTON AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST
ERYTHROCYTIC STAGE OF PLASMODIUM VIVAX |
| |
| Luo Shuhong.PRODUCTON AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST
ERYTHROCYTIC STAGE OF PLASMODIUM VIVAX[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),1995(3). |
| |
| Authors: | Luo Shuhong |
| |
| Abstract: | With Percoll
density gradient centrifugation, the erythrocytes infected P. vivax fromvolunteers were
separated as immunogen and immunized Bale/c mice,in which spleen cellswere fused with
mouse mveloma cells SP2/0.Monoclonal antibodies(McAb)secreted by hy-bridomas cell lines
3D5,4B2,4F11.5C7.8E3 were screened by EIJISA and cloned.In IFAand IPA staining assay,McAb
4B2 and 8E3 showed strong positive reaction with P. vivaxblood stage parasites,The
cytopla2m of the parasites showed bright green fluorescencewhereas the nucleus did not.The
parasites were clearly visible as dark brown bodies afterimmunoperoxidase staining. McAb
4B2 and 8E3 did not show cross-reaction with the erythrocytic stages of P.
yoelii. |
| |
| Keywords: | ntibodies monoclonal plasmodium vivax hybridomas: immunoen-zyme techniques mice |
| 本文献已被 CNKI 等数据库收录！ |
