pBMP/MPTCP复合人工骨治疗骨缺损的实验研究

将猪骨形态发生蛋白 (BMP)与磁性多孔磷酸三钙 (MPTCP)结合制成人工骨 ,手术造成兔桡骨中段 1cm缺损 ,分别嵌入pBMP/MPTCP(A组 ) ,自体红骨髓 /多孔磷酸三钙 (BM/PTCP) (B组 )及pBMP/PTCP(C组 )。观察术后一般情况、免疫球蛋白及补体、大体标本骨痂量、X线、组织形态学、骨钙素检测、新骨形成面积比、生物力学实验等指标。实验结果显示 ,三组骨缺损均有不同程度修复且生物相容性良好 ,但A组较另二组有更多新骨形成及更好的力学性能 (P <0 .0 1) ;B ,C二组间差异无显著性。作者认为pBMP/MPTCP有以下特点 :促进新骨形成能力强 ,生物相容性良好 ,有良好的填充作用 ,可在体内逐渐降解

Experimental study of pBMP/MPTCP in bone defects therapy

To develop a new substitute of bone graft, which is composed of porcine bone morphogenetic protein (pBMP) and the magnetic porous tricalcium phosphate (MPTCP), we investigated the effects of pBMP/MPTCP (Group A), autologous red bone marrow/porus tricalcium phosphate (BM/PTCP) (Group B), pBMP/PTCP(Group C) on the repair of bone defects created surgically in rabbits . By gross inspection, roentgenography examination, histological examination, immunohistochemistry test of osteocalcim, quantitive histological measurement of new bone formation and biomechanical test, three kinds of substitutes were proved to be biocompatible. Group A induced more new bone formation than other two groups, and showed a better biomechanical function (P<0.01). It is believed that pBMP/MPTCP has characteristics of strong osteoinductive capacity, good biocompatibility, excellent filling of bone defects and gradual biodegradation.