| 新型冠状病毒SARS-CoV-2特异性可检测靶点的确认与重叠PCR合成 |
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| 引用本文： | 肖正午,肖莉,传军,李亚梅,彭壮,林丽美,李凯,戴爱国,庹勤慧,廖端芳.新型冠状病毒SARS-CoV-2特异性可检测靶点的确认与重叠PCR合成[J].南华大学学报(医学版),2020(6):641-646. |
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| 作者姓名： | 肖正午 肖莉 传军 李亚梅 彭壮 林丽美 李凯 戴爱国 庹勤慧 廖端芳 |
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| 作者单位： | 湘产大宗药材品质评价湖南省重点实验室,湖南 长沙 410208;苏州大学附属第二医院, 江苏 苏州 215004;人和未来生物科技长沙有限公司,湖南 长沙 410000;湖南中医药 大学医学院呼吸疾病研究室,湖南 长沙 410000 |
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| 摘 要： | 高敏感的检测试剂盒的基因检测,是防控新冠疫情的关键手段之一。鉴于目前国内外新型冠状病毒(SARS-CoV-2)检测试剂盒在敏感性方面存在的不足,我们认为与其所检测靶点数量的局限性有关。本研究结合生物信息学分析和采用重叠PCR合成策略,获得了可用于新型冠状病毒检测的8个新靶点。这些靶点主要呈现为两个方面的特点:一是增加了扩增引物的SARS-CoV-2特异性；二是增强了适宜于TaqMan探针检测的SARS-CoV-2特异性。这些新的靶点有望成为未来SARS-CoV-2高灵敏度检测及试剂盒开发的重要对象。
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| 关 键 词： | SARS-CoV-2 检测靶点 PCR |
| 收稿时间： | 2020/10/8 0:00:00 |
| 修稿时间： | 2020/10/12 0:00:00 |
Identification and overlapping PCR synthesis of detectable SARS-COV-2 specific gene fragments |
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| XIAO Zhengwu,XIAO Li,CHUAN Jun,LI Yamei,PENG Zhuang,LIN Limei,LI Kai,DAI Aiguo,TUO Qinhui,LIAO Duanfang.Identification and overlapping PCR synthesis of detectable SARS-COV-2 specific gene fragments[J].Journal of Nanhua University(Medical Edition),2020(6):641-646. |
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| Authors: | XIAO Zhengwu XIAO Li CHUAN Jun LI Yamei PENG Zhuang LIN Limei LI Kai DAI Aiguo TUO Qinhui LIAO Duanfang |
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| Institution: | Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province,Changsha 410208, Hunan,China;The Second Affiliated Hospital of Soochow University,Suzhou 215004,Jiangsu, China;GeneTalks Biotech Co.,Ltd.,Changsha 410010,Hunan,China;Department of Respiratory Diseases,School of Medicine,Hunan University of Chinese Medicine, Changsha 410208,Hunan,China |
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| Abstract: | Detection kits with high sensibility are one of the key tools for COVID-19 control. The relatively low sensitivity of SARS-COV-2 detection kits presently used is partially related to the limited number of the viral gene fragments targeted. In this study, eight novel detection targets of SARS-COV-2 were obtained by combining bioinformatics analysis and overlapping PCR synthesis strategy. These targets are characterized by two aspects:SARS-COV-2 specificity of the amplified primers and being suitable for TaqMan probe detection. These new targets add important resource for high sensitivity detection and kit development of SARS-COV-2 in the future. |
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| Keywords: | SARS-CoV-2 detectable targets PCR |
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