姜黄素对脑缺血再灌注自发性高血压大鼠认知功能的影响

目的：探讨姜黄素对脑缺血再灌注自发性高血压（SH）大鼠认知功能的影响。方法:选取75只大鼠随机分为假手术组、模型组和实验组，每组25只；模型组和实验组构建脑中动脉栓塞（MCAO）模型，假手术组不阻塞大脑中动脉血供；实验组于术前30 min及再灌注后6 h腹腔注射姜黄素100 mg/kg，假手术组和模型组注射等量生理盐水。检测大鼠认知功能、缺血侧海马区神经元凋亡及相关蛋白的表达。结果：缺血再灌注2~72 h，与假手术组比，模型组神经元凋亡细胞数量增多，而实验组较模型组减少；模型组穿台次数减少，实验组多于模型组；模型组逃避潜伏期延长，实验组短于模型组；模型组缺血侧海马区磷脂酰肌醇3激酶（PI3K）、磷酸化蛋白激酶B（p-AKT）、半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）蛋白含量高于假手术组，实验组PI3K、p-AKT蛋白含量高于模型组，Caspase-3蛋白低于模型组（均P<0.05）。结论:姜黄素可减轻脑缺血再灌注SH大鼠神经元凋亡程度，改善认知功能，其机制与上调PI3K、p-AKT蛋白表达及下调Caspase-3蛋白表达相关。

Effect of Curcumin on Cognitive Function in Spontaneously Hypertensive Rats with Cerebral Ischemia Reperfusion

To investigate the effect of curcumin on cognitive function in spontaneously hypertensive (SH) rats with cerebral ischemia reperfusion. Methods:A total of 75 cases of SH rats were selected and randomly divided into a sham operation group, a model group and a test group, with 25 cases in each group. The middle cerebral artery occlusion (MCAO) model was established in model group and test group, while the sham operation group did not block the blood supply of the middle cerebral artery; test group received intraperitoneal injection of curcumin 100 mg·kg-1 at 30 min before operation and 6 h after reperfusion, sham operation group and model group were given intraperitoneal injection of the same amount of normal saline. The cognitive function, neuronal apoptosis and the expression of related proteins in the hippocampus of the ischemic side were detected. Results:After 2 h-72 h of cerebral ischemia and reperfusion, compared with sham operation group, the number of apoptotic neurons in model group increased, while the test group was less than the model group; the number of crossings reduced, and the test group was more than the model group; the escape latency prolonged, and the test group was shorter than the model group; the content of phosphatidylinositol 3 kinase (PI3K), phosphorylated protein kinase B (p-AKT) and cysteine aspartate protease-3 (Caspase-3) in ischemic side hippocampus of rats in model group were higher than those in sham operation group, while the content of PI3K and p-AKT in test group were higher than model group, Caspase-3 was lower than model group (all P<0.05). Conclusion:Curcumin can relieve neuronal apoptosis degree and improves cognitive function in SH rats with cerebral ischemia and reperfusion, and the mechanism is related to the up-regulation of PI3K, p-AKT protein and down-regulation of Caspase-3 protein expression.