Heme Oxygenase-1 Regulates Myeloid Cell Trafficking in AKI |
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Authors: | Travis D. Hull Ahmed I. Kamal Ravindra Boddu Subhashini Bolisetty Lingling Guo Cornelia C. Tisher Sunil Rangarajan Bo Chen Lisa M. Curtis James F. George Anupam Agarwal |
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Affiliation: | *Nephrology Research and Training Center.;‡Division of Nephrology, Department of Medicine, and;†Division of Cardiothoracic Surgery, Department of Surgery, University of Alabama at Birmingham, Birmingham, Alabama; and;§Birmingham Veterans Administration Medical Center, Birmingham, Alabama |
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Abstract: | Renal ischemia-reperfusion injury is mediated by a complex cascade of events, including the immune response, that occur secondary to injury to renal epithelial cells. We tested the hypothesis that heme oxygenase-1 (HO-1) expression, which is protective in ischemia-reperfusion injury, regulates trafficking of myeloid-derived immune cells in the kidney. Age-matched male wild-type (HO-1+/+), HO-1–knockout (HO-1−/−), and humanized HO-1–overexpressing (HBAC) mice underwent bilateral renal ischemia for 10 minutes. Ischemia-reperfusion injury resulted in significantly worse renal structure and function and increased mortality in HO-1−/− mice. In addition, there were more macrophages (CD45+ CD11bhiF4/80lo) and neutrophils (CD45+ CD11bhi MHCII− Gr-1hi) in HO-1−/− kidneys than in sham and HO-1+/+ control kidneys subjected to ischemia-reperfusion. However, ischemic injury resulted in a significant decrease in the intrarenal resident dendritic cell (DC; CD45+MHCII+CD11bloF4/80hi) population in HO-1−/− kidneys compared with controls. Syngeneic transplant experiments utilizing green fluorescent protein–positive HO-1+/+ or HO-1−/− donor kidneys and green fluorescent protein–negative HO-1+/+ recipients confirmed increased migration of the resident DC population from HO-1−/− donor kidneys, compared to HO-1+/+ donor kidneys, to the peripheral lymphoid organs. This effect on renal DC migration was corroborated in myeloid-specific HO-1−/− mice subjected to bilateral ischemia. These mice also displayed impaired renal recovery and increased fibrosis at day 7 after injury. These results highlight an important role for HO-1 in orchestrating the trafficking of myeloid cells in AKI, which may represent a key pathway for therapeutic intervention. |
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Keywords: | ARF heme oxygenase macrophages |
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