营养干预对高+Gy暴露小鼠脑功能及相关生化指标的影响

目的研究高 +Gy暴露对小鼠迷宫行为和脑功能相关生化指标的影响和营养干预的效果。 方法昆明种雄性小鼠经迷宫训练后分为对照组 (A组 )、+Gy组 (B组 )、+Gy营养干预组 (C组 ) ;A组不予 +Gy暴露 ;B组、C组均给予 + 1 0Gy暴露 8min ,B组暴露前 3h蒸馏水灌胃 ;C组实验前 1d给予强化VitB6 的饮水 ,暴露前 3h混合氨基酸液灌胃 ;3组动物均记录迷宫实验成绩 ,检测血清氨基酸f、脑组织单胺类递质含量和γ 谷氨酰转移酶活性。结果 +Gy暴露后迷宫实验成绩下降 ,并有行为异常 ,脑内NE明显降低 ,其他单胺类递质均显著升高 (P <0 0 5 ) ,表明脑功能受影响 ;营养干预后迷宫行为改善 ,生化指标改变有所缓解。结论高 +Gy暴露会引起中枢递质相关的生化营养物质代谢紊乱并影响脑功能 ,营养干预对此有一定的防护效果

Effects of Nutritional Intervention on Brain Functions and Its Related Biochemical Indexes in Mice Exposed to High +Gy

Objective: To study the effects of nutritional intervention on changes of monoamines in rats brain, certain amino acids in rats serum and maze behaviors caused by high +Gy exposure. Method: The mice were arranged into control group (Group A), +Gy group (Group B) and +Gy nutritional intervention group (group C). Group A was not exposed to +Gy. Both Group B and group C were exposed to +10 Gy for 8 min. Three hours before +Gy exposure distilled water was given to mice in group B by gavage. The day before exposure pyridoxal fortified water was given and 3 h before exposure mixed amino acids solution were given by gavage to group C. Maze test scores were recorded for all groups. After the maze test was completed, blood was collected through the eyes for serum amino acids and brain tissue was collected by decollation for monoamines and gamma-glutamyl transferase (GGT) activity determinations. Result: After +Gy exposure maze test scores and brain NE concentrations decreased and abnormal behaviors were found. While other monoamine transmitters increased significantly. In group C maze behaviors improved and biochemical changes induced by +Gy exposure alleviated. Conclusion: High +Gy exposure can induce changes of neural transmitters coming from nutritional metabolites in central nervous system. As a result, brain functions are affected. Nutritional intervention can alleviate this negative effect to some extent.