| c-myc反义寡核苷酸对人恶性黑素瘤细胞c-myc mRNA表达及其蛋白水平的影响 |
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| 引用本文： | 张江安,谭升顺,王宏瑾,彭振辉. c-myc反义寡核苷酸对人恶性黑素瘤细胞c-myc mRNA表达及其蛋白水平的影响[J]. 中国皮肤性病学杂志, 2005, 19(8): 458-459,470 |
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| 作者姓名： | 张江安 谭升顺 王宏瑾 彭振辉 |
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| 作者单位： | 1. 西安交通大学第二医院皮肤科,陕西,西安,710004
2. 郑州大学第四附属医院皮肤科,河南,郑州,454006 |
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| 摘 要： | 目的研究c-myc反义寡核苷酸(ASODN)对人恶性黑素瘤细胞c-myc mRNA表达及c-myc蛋白水平的影响。方法设空白和混杂链(SODN)对照,人工合成c-myc ASODN,以30μmol/L ASODN转染人恶性黑素瘤细胞株A375,分别用原位杂交、免疫组化SABC法检测c-myc mRNA的表达及其蛋白水平。结果转染24 h后,ASODN组细胞c-myc mRNA较空白对照组、SODN组均显著下调(P均<0.01);转染48 h后,与空白对照组、SODN组相比,ASODN组细胞的c-myc蛋白水平均显著下降(P均<0.01)。结论c-myc ASODN可下调A375细胞c-myc mRNA的表达和c-myc蛋白水平。
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| 关 键 词： | 原癌基因 寡核苷酸,反义 黑素瘤 |
| 文章编号： | 1001-7089（2005）08-0458-03 |
| 收稿时间： | 2005-05-21 |
| 修稿时间： | 2005-05-21 |
Effects of c-myc Antisense Oligodexynucleotide on the Expression of c-myc mRNA and Protein in Melanoma Cells |
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| Zhang JiangAn;Tan ShengShun;Wang HongJin;Peng ZheHui. Effects of c-myc Antisense Oligodexynucleotide on the Expression of c-myc mRNA and Protein in Melanoma Cells[J]. The Chinese Journal of Dermatovenereology, 2005, 19(8): 458-459,470 |
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| Authors: | Zhang JiangAn Tan ShengShun Wang HongJin Peng ZheHui |
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| Abstract: | Objective To study the effects of c-myc antisense oligodexynucleotide (ASODN) on the expression of c-myc mRNA and its protein in melanoma cells.Methods c-myc ASODN was synthesized and transfected into melanoma cell line A375. c-myc mRNA and protein were detected by in situ hybridization and immunohistochemistry, respectively.Results At 24h after incubation, the expression of c-myc mRNA was significantly lower in the ASODN group than that in SODN group as well as that in the control group(P<0.01);compared to those of empty control and SODN group,the level of c-myc protein in the ASODN group significantly decreased at 48h after incubation(P<0.01).Conclusion c-myc ASODN can down-regulate the expression of c-myc mRNA and its protein in A375 cells. |
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| Keywords: | Protooncogene Oligonucleotide antisense Melanoma |
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