| 高效液相色谱法测定防感喷雾剂中次野鸢尾黄素的含量 |
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| 引用本文： | 李甲英,翟玉,王泽锋,李姝梅,韦静,李安华. 高效液相色谱法测定防感喷雾剂中次野鸢尾黄素的含量[J]. 儿科药学杂志, 2012, 18(10): 43-45 |
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| 作者姓名： | 李甲英 翟玉 王泽锋 李姝梅 韦静 李安华 |
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| 作者单位： | 1. 云南中医学院,云南昆明,650500
2. 云南省红河学院,云南红河,661100 |
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| 基金项目： | 云南省科技项目;项目编号2008IF014 |
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| 摘 要： | 目的:建立高效液相色谱( HPLC)测定防感喷雾剂中次野鸢尾黄素含量的方法.方法:采用HPLC进行测定,色谱柱为Agilent C18柱(250 mm×4.6 mm,5 μm)；流动相为甲醇-0.2％磷酸(60∶40)；检测波长266 nm；流速1.0 mL/min；柱温30℃.结果:次野鸢尾黄素在0.026～0.260 mg/mL范围内线性关系良好(r=1.000),平均加样回收率为97.12％,RSD为1.85％.结论:本方法简便、准确、可靠,可用于防感喷雾剂中次野鸢尾黄素的含量测定.
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| 关 键 词： | 高效液相色谱法 次野鸢尾黄素 防感喷雾剂 |
Determination of Irisflorentin in Fanggan Spray by HPLC |
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| Li Jiaying , Zhai Yu , Wang Zefeng , Li Shumei , Wei Jing , Li Anhua. Determination of Irisflorentin in Fanggan Spray by HPLC[J]. Journal of Pediatric Pharmacy, 2012, 18(10): 43-45 |
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| Authors: | Li Jiaying Zhai Yu Wang Zefeng Li Shumei Wei Jing Li Anhua |
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| Affiliation: | 1(1.Yunnan University of Traditional Chinese Medicine,Yunnan Kunming 650500,China;2.Honghe College of Yunnan Province,Yunnan Honghe 661100,China) |
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| Abstract: | Objective: To establish a method for the determination of irisflorentin in Fanggan spray by HPLC.Methods: HPLC method was used to carry on the determination;chromatographic bar was Agilent C18(250 mm×4.6 mm,5 μm).The mobile phase was methanol-0.2% phosphoric acid(60∶40).The flow rate was 1.0 mL/min with detection wavelength of 266 nm.Column temperature was set at 30 ℃.Results: The irisflorentin had a good linear relation between 0.026~0.260 mg/mL(r=1.000).This average recovery was 97.12% and RSD was 1.85%.Conclusions: This method is simple,accurate and reliable.It can be used as a method for determination of irisflorentin in Fanggan spray. |
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| Keywords: | HPLC Irisflorentin Fanggan spray |
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