Inflammation induced by increased frequency of intermittent hypoxia is
attenuated by tempol administration |
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Authors: | J Zhang L Zheng J Cao B Chen D Jin |
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Institution: | Department of Respiratory, Tianjin Medical University General Hospital, Tianjin, China |
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Abstract: | The levels of serum inflammatory cytokines and the activation of nuclear factor kappa
B (NF-κB) and hypoxia inducible factor-1α (HIF-1α) in heart tissues in response to
different frequencies of intermittent hypoxia (IH) and the antioxidant tempol were
evaluated. Wistar rats (64 males, 200-220 g) were randomly divided into 6
experimental groups and 2 control groups. Four groups were exposed to IH 10, 20, 30,
or 40 times/h. The other 2 experimental groups were challenged with IH (30 times/h)
plus tempol, either beginning on day 0 (IH30T0) or on day 29 (IH30T29). After 6 weeks
of challenge, serum levels of tumor necrosis factor (TNF)-α, intracellular adhesion
molecule (ICAM)-1, and interleukin-10 were measured, and western blot analysis was
used to detect NF-κB p65 and HIF-1α in myocardial tissues. Serum levels of TNF-α and
ICAM-1 and myocardial expression of NF-κB p65 and HIF-1α were all significantly
higher in IH rats than in controls (P<0.001). Increased IH frequency resulted in
more significant changes. Administration of tempol in IH rats significantly reduced
levels of TNF-α, ICAM-1, NF-κB and HIF-1α compared with the non-tempol-treated group
(F=16.936, P<0.001). IH induced an inflammatory response in a frequency-dependent
manner. Additionally, HIF-1α and NF-κB were increased following IH administration.
Importantly, tempol treatment attenuated this effect. |
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Keywords: | Obstructive sleep apnea Intermittent hypoxia Inflammation Nuclear factor kappa B Antioxidant |
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