Vertical transmission of human T-cell leukemia virus type I (HTLV-I): Detection of proviral DNA in HTLV-I carrier gravida |
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Authors: | Fumitaka Saji Yoshihiro Tokugawa Shoji Kamiura Yoshihiro Samejima Kazutomo Ohashi Chihiro Azuma Osamu Tanizawa |
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Institution: | (1) Department of Obstetrics and Gynecology, Osaka University Medical School, 1-1-50 Fukushima, Fukushima-ku, 553 Osaka, Japan |
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Abstract: | The seroprevalence rate of human T-cell leukemia virus type I (HTLV-I) in pregnant women in the Osaka district was determined by enzyme-linked immunosorbent assay and Western blot analysis. Twenty-one (1.0%) of 2192 samples tested were positive for both assays and the seropositive parturients were found to be integrated with HTLV-I proviral DNA in their mononuclear cells by a DNA dot blot hybridization assay using HTLV-I DNA probe or by a selective DNA amplification technique using the polymerase chain reaction (PCR). On the other hand, proviral DNA was not detected in cord blood of the neonates born to the carrier mothers, indicating that transplacental infection of HTLV-I during pregnancy could be excluded. The results support the hypothesis that postpartum infection via breast milk plays a significant role among the possible perinatal transmission routes. |
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Keywords: | Retrovirus human T-cell leukemia virus type I (HTLV-I) perinatal infection proviral DNA DNA blot hybridization polymerase chain reaction (PCR) |
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