| Establishment of Mus Skin Photo-damage Model by 8-MOP plus UVA Irradiation |
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| 作者姓名： | 梁虹 李家文 张璃 |
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| 作者单位： | Department of Dermatology Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Dermatology Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Dermatology No. 1 Hospital Affiliated to Wenzhou Medial College，Wuhan 430022 China，Wuhan 430022 China，Wenzhou 325000 China |
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| 摘 要： | To establish a simple and reliable animal model of skin photo-damage, 20 mice were treated with 8-MOP and exposed to UVA (UVA 320–400 nm) for 24 h. After irradiation, the structure of the epidermis and dermis, collagen fibers, elastic fibers were observed by using HE staining and Weigert technique and compared with the normal controls. The acanthosis and epidemis proliferation with accompanying hyperkeratosis and parakeratosis were observed. Inflammatory infiltration was noted in the dermis. The elastic fibers became coarse, irregularly arranged and clustered, with their number increased. The collagen fibers showed obvious degeneration and some amorphous materials could also be observed. The blood vessels were irregularly dilated and vascular walls were thickened, with infiltration of inflammatory cells. It is concluded that murine photodamage model can be quickly, conveniently and reliably established by means of 8-MOP/UVA.
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| 关 键 词： | 皮肤损伤 8-MOP 紫外线照射 动物模型 |
| 收稿时间： | 2007-11-09 |
Establishment of mus skin photo-damage model by 8-MOP plus UVA irradiation |
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| Liang Hong,Li Jiawen,Zhang Li.Establishment of Mus Skin Photo-damage Model by 8-MOP plus UVA Irradiation[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2007,27(6):742-744. |
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| Authors: | Liang Hong Li Jiawen Zhang Li |
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| Institution: | 1. Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
2. Department of Dermatology, No. 1 Hospital Affiliated to Wenzhou Medial College, Wenzhou 325000, China |
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| Abstract: | To establish a simple and reliable animal model of skin photo-damage, 20 mice were treated with 8-MOP and exposed to UVA (UVA 320-400 nm) for 24 h. After irradiation, the structure of the epidermis and dermis, collagen fibers, elastic fibers were observed by using HE staining and Weigert technique and compared with the normal controls. The acanthosis and epidemis proliferation with accompanying hyperkeratosis and parakeratosis were observed. Inflammatory infiltration was noted in the dermis. The elastic fibers became coarse, irregularly arranged and clustered, with their number increased. The collagen fibers showed obvious degeneration and some amorphous materials could also be observed. The blood vessels were irregularly dilated and vascular walls were thickened, with infiltration of inflammatory cells. It is concluded that murine photodamage model can be quickly, conveniently and reliably established by means of 8-MOP/UVA. |
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| Keywords: | skin photodamage 8-MOP/UVA animal model |
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