糖尿病大鼠周围神经NGF的动态表达及巴曲酶对其影响

目的探讨神经生长因子（NGF）在糖尿病周围神经病（DPN）大鼠中的动态变化规律,初步证实巴曲酶对NGF的影响并探讨其机制,为临床上治疗DPN提供理论依据。方法将大鼠腹腔注射链脲佐菌素制作实验性糖尿病模型,再分别于成模后2个月和3个月时腹腔注射巴曲酶进行干预。通过免疫组织化学和原位杂交双重检测NGF在坐骨神经中的表达。结果DM造模后2个月时坐骨神经中NGF的含量明显减少,且随时间变化有统计学差异,而巴曲酶治疗后NGF的表达明显增加。结论DPN大鼠坐骨神经中NGF表达减少可能参与DPN的发病机制。巴曲酶对DPN有保护作用,其机制可能包括对NGF的调节。

The impact of Batroxobin on The dynamic expression of nerve growth factor in peripheral nerve of diabetic rats

Objective To investigate the dynamic expression of nerve growth factor(NGF)in diabetic pe- ripheral neuropathy(DPN)rats and to confirm the impact of Batroxobin on NGF tentatively and the mechanism in order to furnish the theory foundation for the therapy of DPN clinically.Methods Through the peritoneal injec- tion of streptozocin to make the model of experimental diabetes,we ejected Batroxobin in abdominal cavity of rats respectively 2 months and 3 months after the formation of diabetes model for intervention.We took immunohis- tochemistry and hybridization in situ to detect the expression of NGF in ischiadic nerve of the rats.Results The content of NGF in ischiadic nerve decreased obviously 2 months after the formation of diabetes model and it had time-varying statistics diversity,however the expression of NGF was markedly increased after the treatment of Ba- troxobin.Conclusion The decreased expression of NGF in ischiadic nerve in DPN rats might play an important role in the pathogenesis of DPN.Treatment with Batroxobin is beneficial to the prevention of DPN,possibly through the regulation of the expression level of NGF in the sciatic nerves of the DPN rats.