IL-4及CD40 mAb对肾小管上皮细胞RANTES表达的影响

目的:观察小鼠肾小管上皮细胞(TEC)在IL-4及CD40激活状态下激活正常T细胞表达和分泌因子(RANTES)分泌的变化。方法:刺激原代培养的小鼠肾小管上皮细胞,检测RANTES的表达,流式细胞仪检测CD40表达。结果:(1)常规培养下,小鼠TEC可表达一定量的CD40,用IL-4刺激TEC 24 h,细胞表面CD40 MFI 显著高于对照组(P<0.01)。(2)常规培养下,小管上皮细胞仅分泌极少量的RANTES(14.78 ng/L±2.20 ng/L),在IL-4刺激或CD40mAb激活后TEC RANTES蛋白分泌分别为43.61±13.73和73.77±4.28,显著高于对照组(P<0.01)。用IL-4刺激肾小管细胞CD40表达的同时,加入纯化的CD40mAb激活CD40受体,TEC RANTES 的合成、分泌显著高于对照组(131.77±41.87,P<0.01),与单纯IL-4刺激组及CD40mAb刺激组比较差异显著(均P<0.01)。(3)用IL-4、CD40mAb及 IL-4+CD40mAb刺激TEC细胞24 h,各刺激组RANTES mRNA表达显著高于对照组(P<0.05)。结论:IL-4及CD40-CD40L共刺激信号可调控RANTES合成及分泌,参与TEC炎症过程。

Effect of IL-4,CD40L on RANTES production in murine renal tubular epithelial cells

AIM: To investigate the effect of IL-4, CD40L on RANTES production in murine renal tubular epithelial cells (TEC). METHODS: TEC were obtained from mouse, expression of RANTES and CD40 on TEC were measured. RESULTS: (1) Activation of TEC with IL-4 resulted in significant increase in CD40 expression (P＜0.01).(2) A little RANTES was detectable in supernatants without stimulation. TEC stimulated with either cytokine IL-4 or CD40mAb resulted in strong induction of RANTES production up to 43.61±13.73 or 73.77±4.28(ng/L), respectively. The differences of RANTES between two stimulation groups and that in medium were statistically significant (P<0.01). TEC stimulated with IL-4 and CD40mAb produced more RANTES than that in medium (P<0.01), which was higher than that with single stimulation (P<0.01). (3) TEC stimulated with IL-4 or CD40 activation or combined stimulation of IL-4 and CD40mAb resulted in increase in levels of RANTE mRNA, which were higher than that in medium. CONCLUSION: Co-stimulation of TEC by IL-4 and CD40mAb up-regulated the RANTES production, suggesting the RANTES may participate in the inflammation of TEC.