A novel fixative for immunofluorescence staining of CD133-positive glioblastoma stem cells |
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Authors: | Sherman Jonathan H Redpath Gerard T Redick Jan A Purow Benjamin W Laws Edward R Jane John A Shaffrey Mark E Hussaini Isa M |
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Institution: | Department of Neurological Surgery, University of Virginia, Charlottesville, Virginia, USA. jsherman0620@gmail.com |
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Abstract: | Isolation of glioblastoma stem cells requires incubation of tumor cells in a neural stem cell media. Neurospheres containing these glioblastoma stem cells are formed after approximately a five-day period. These cells can then be analyzed for the presence of stem cell markers. Immunofluorescence staining for these markers can serve as a valuable tool for analyzing the intact neurosphere directly in stem cell media. Here we present the use of a novel fixative (1,4-benzoquinone) for immunoflourescence staining of neurospheres. |
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