甲状腺肿瘤细胞中P53、P63蛋白表达的比较研究

目的探讨P5 3和P6 3蛋白在甲状腺肿瘤各型细胞中的表达情况 ,并观察它们的相似及差异性。方法采用免疫组织化学 (EnvisionTM)法检测 6 0例甲状腺肿瘤中P5 3和P6 3蛋白的表达。结果P5 3蛋白在甲状腺腺瘤及腺癌中的阳性表达率分别为 0 0 0 % (0 /30 )和 2 0 0 % % (6 /30 ) ,两者比较差异有显著意义 (P <0 0 5 ) ;P6 3蛋白在甲状腺腺瘤及腺癌中的阳性表达率分别为 4 6 7% (14 /30 )和 2 0 0 % (6 /30 ) ,两者比较差异有显著意义 (P <0 0 5 ) ;P5 3在甲状腺乳头状腺瘤及滤泡状腺瘤中均不表达 ,而在甲状腺乳头状腺癌及滤泡状腺癌中的阳性表达率分别为 13.3% (2 /15 )和 33.3% (5 /15 ) ;P6 3蛋白在甲状腺乳头状腺瘤及滤泡状腺瘤中的阳性表达率分别为 6 0 .0 % (9/15 )和 33.3% (5 /15 ) ,而在甲状腺乳头状腺癌及滤泡状腺癌中的阳性表达率均为 2 0 .0 % (3/15 )。结论P5 3和P6 3蛋白在甲状腺肿瘤细胞中表达的差异 ,可能提示P5 3和P6 3蛋白在肿瘤抑制和组织发育中起着不同的作用。

Expression of P53 and P63 proteins in thyroid neoplasms

Objective To investigate the expression of P53 and P63 protein in thyroid neoplasma, the comparability and the differentiability. Methods The expression of P53 and P63 were examined by immunohistochemical (Envision TM ) in 60 cases of thyroid neoplasm. Results The positive rates of P53 in thyroid asenoma and thyroid carcinoma were 0 and 20.0% respectively (P<0.05). The positive rates of P53 protein were 0 in thyroid papillary asenoma and follicular adenoma. The positive rate of P53 protein in thyroid papillary carcinomas and follicular carcinomas were 13.3% and 33.3% respectively. The positive rate of P63 protein in thyroid papillary carcinomas and follicular carcinomas were both 20.0%. Conclusion The expression differences of P53 and P63 protein in thyroid neoplasms cells indicated that the proteins play different role to against tumours.