|
|||||
|
|
| HPLC-DAD/ELSD、UPLC-MS/MS分析复方五仁醇胶囊中皂苷类成分 | |
| 引用本文： | 窦志华,罗 琳,居文政,丁安伟. HPLC-DAD/ELSD、UPLC-MS/MS分析复方五仁醇胶囊中皂苷类成分[J]. 药学与临床研究, 2010, 18(1): 38-41 |
| 作者姓名： | 窦志华 罗 琳 居文政 丁安伟 |
| 作者单位： | 1. 南通市第三人民医院,南通,226006 2. 南通大学,南通,226006 3. 南京中医药大学附属医院,南京,210029 4. 南京中医药大学,南京,210029 |
| 基金项目： | 江苏省中医药局中医药科学技术研究专项课题 |
| 摘 要： | 目的：分析复方五仁醇胶囊中皂苷类成分。方法：分别采用HPLC—DAD／ELSD、UPLC—MS／MS检测，通过与对照品色谱图比对及比较制剂和对照品提取离子流色谱图（EIC）。并通过质谱图相关离子峰分析，鉴别制剂中的皂苷类成分。结果：HPLC—DAD／ELSD检测出三七皂苷R1、人参皂苷Rg1及人参皂苷Rb1，未检测到柴胡皂苷A和D；UPLC—MS／MS除检测到上述3种三七皂苷类成分外，还检测到柴胡皂苷D，但柴胡皂苷A仍未检测到。结论：复方五仁醇胶囊中存在三七和柴胡所含皂苷类成分。 |
| 关 键 词： | 高效液相色谱-二极管阵列／蒸发散射检测器 超高效液相色谱-串联质谱 人参皂苷 柴胡皂苷 复方五仁醇胶囊 |
| 收稿时间： | 2009-10-11 |
Analysis of Saponins in Compound Wurenchun Capsula by HPLC-DAD/ELSD and UPLC-MS/MS |
|
| DOU Zhi-hu,LUO Lin,JU Wen-zheng and DING An-wei. Analysis of Saponins in Compound Wurenchun Capsula by HPLC-DAD/ELSD and UPLC-MS/MS[J]. Pharmacertical and Clinical Research, 2010, 18(1): 38-41 | |
| Authors: | DOU Zhi-hu LUO Lin JU Wen-zheng DING An-wei |
| Affiliation: | DOU Zhi-hua1,LUO Lin2,JU Wen-zheng3,DING An-wei4 1Nantong Third People's Hospital,Nantong 226006,2Nantong University,China,3Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,4Nanjing University of Chinese Medicine |
| Abstract: | Objective:To analyze saponins in Compound Wurenchun Capsula.Methods:Saponins were identified by comparing the chromatograms detected by HPLC-DAD/ELSD and the extracted ion chromatograms(EIC) detected by UPLC-MS/MS of Compound Wurenchun Capsula with thoses of reference substances,correlated ion peaks in mass-spectrogram were analyzed at the same time.Results:Notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 were detected by HPLC-DAD/ELSD,but none of saikosaponin A or D was discovered through this method... |
| Keywords: | HPLC-DAD/ELSD UPLC-MS/MS Ginsenoside Saikosaponin Compound Wurenchun Capsula |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《药学与临床研究》浏览原始摘要信息 | |
| 点击此处可从《药学与临床研究》下载全文 | |