骨髓间充质干细胞联合丹红注射液干预脑梗死组织GAP-43、Bcl-2的表达

背景：丹红注射液能够清除自由基和抑制脂质过氧化反应,能够改善中枢神经系统梗死后损伤区的微环境。目的：探讨骨髓间充质干细胞移植联合丹红注射液对大鼠脑梗死组织GAP-43、Bcl-2表达的影响。方法：选取Wistar大鼠60只,建立大脑中动脉闭塞模型,随机分为对照组、骨髓间充质干细胞组和联合治疗组。对照组尾静脉注射PBS、骨髓间充质干细胞组尾静脉注射2.5×109L-1的骨髓间充质干细胞悬液、联合治疗组联合注射2mL/kg丹红注射液＋2.5×109L-1的骨髓间充质干细胞悬液,均连续5d,1次/d。结果与结论：移植后24h,3d各组间大鼠神经功能障碍评分差异无显著性意义（P〉0.05）,移植后1周、2周,联合治疗组大鼠神经功能障碍评分低于骨髓间充质干细胞组及对照组（P〈0.05）;在联合治疗组中,GAP-43、Bcl-2的表达明显高于骨髓间充质干细胞组及对照组（P〈0.05）。说明骨髓间充质干细胞移植与丹红注射液联合应用可明显促进脑梗死局部GAP-43、Bcl-2的表达,对大鼠脑梗死细胞凋亡具有明显抑制作用。

Effect of bone marrow mesenchymal stem cells combined with Danhong injection on expression of GAP-43 and Bcl-2 after cerebral infarction

BACKGROUND： Danhong injection, scavenging free radicals and inhibiting lipid peroxidation, can improve microenvironment injury after cerebral infarction. OBJECTIVE： To explore the influence of bone marrow mesenchymal stem cells combined with Danhong injection on expression of GAP-43 and Bcl-2 after cerebral infarction in rats. METHODS： Sixty Wistar rats were selected to prepare models of cerebral infarction by middle cerebral artery occlusion and then randomly divided into control group, bone marrow mesenchymal stem cell group, and combination group. Control group received tail vein injection of PBS. Bone marrow mesenchymal stem cell group received tail vein injection of 2.5×10 9 /L bone marrow mesenchymal stem cell suspension. Combination group received injection of 2.5× 10 9 /L bone marrow mesenchymal stem cell suspension＋2 mL/kg Danhong injection, for 5 consecutive days, once a day. RESULTS AND CONCLUSION： There were no significant differences in the neurological dysfunction scores among the three groups at 24 hour and 3 days after implantation （P〈0.05）. The neurological dysfunction scores in the ombination group were significantly lower than those in the bone marrow mesenchymal stem cell group and control group at 1 and 2 weeks after transplantation （P〈0.05）. In the combination group, GAP-43 and Bcl-2 expression was significantly higher than the bone marrow mesenchymal stem cell group and control group （P〈0.05）. Bone marrow mesenchymal stem cell transplantation combined with Danhong injection can significantly promote the local expression of GAP-43 and Bcl-2 after cerebral infarction, and has obvious inhibitory effects on cell apoptosis in rats with cerebral infarction.