载脂蛋白E基因多态性与早发冠心病的关系及其对血脂的影响

目的 研究载脂蛋白E（apolipoprotein E,apoE)基因多态性与早发冠心病（coronary heart disease,CHD)的相关关系及其对血脂水平的影响。方法 应用聚合酶链反应－限制性片段长度多态性（polymerase chain reaction-restricted fragment hength polymorphism,PCR-RFLP)基因分析方法，测定52例早发CHD、161例迟发CHD患者和180名对照者的apoE基因型；血脂水平按常规方法测定。结果 发现的5种apoE基因型，分别为E3／3、E4／4、E3／2、E4／3及E4／2。早发CHD组和迟发CHD组apoE4/3基因型和ε4等位基因频率均高于对照组（P＜0．01）；进一步对两组CHD患者的apoE多态性进行分析，发现早发组ε4等位基因频率较迟发组为高（P＜0．05）。apoE各等位基因型之间，TC和LDL－C水平之间存在统计学差异（P＜0．05）。结论 apoE基因多态性与早发CHD的发生发展有关并影响血脂的水平。

Association of apolipoprotein E gene polymorphism with early-onset coronary heart disease and its effect on plasma lipid levels

OBJECTIVE: To detect the association of apolipoprotein E(apoE) gene polymorphism with early-onset coronary heart disease(CHD) and to evaluate the effect of apoE gene polymorphism on plasma lipid levels. METHODS: The apoE genotypes of 52 early-onset CHD patients (aged 38-55 years), 161 aged CHD patients and 180 controls were determined by PCR-RFLP. Plasma lipid levels were measured by using routine methods. RESULTS: Five genotypes of apoE, E3/3, E4/4, E3/2, E4/3 and E4/2 were detected in this study. Statistically differences in apoE4/3 and epsilon4 frequencies between early-onset and aged CHD patients and controls were seen (P<0.05). The frequency of epsilon4 in the early-onset CHD patients was higher than that in the aged CHD patients. epsilon4 and epsilon2 alleles also differently affected the plasma total cholesterol and low density lipoprotein cholesterol levels. CONCLUSION: These results suggest that apoE gene polymorphism is in association with the occurrence and development of early-onset CHD and it affects the plasma lipid levels.