组织工程皮肤替代物构建的实验研究

目的探索组织工程皮肤替代物的构建方法。方法采用传代培养的角质形成细胞和成纤维细胞作为种子细胞,I型胶原作为真皮基质,采用气—液界面方式进行培养,用HE染色观察培养物的结构。结果培养的组织工程皮肤替代物具有正常皮肤的形态结构,具有结构致密的真皮和分化良好的表皮。结论本方法可用于活性皮肤替代物的制备。

Experimental study on constructing tissue-engineered skin equivalent

Objective To establish a method of constructing a bilayered living skin equivalent in vitro.Methods Using serially passaged keratinocytes and dermal fibroblasts as seed cells type I collagen as matrix,living skin equivalent has been generated through air-liquid interface culture.Sections of skin equivalent were stained with HE and examined under microscopy.Results Morphology of skin equivalent was similar to that of normal skin.The skin equivalent has density dermis and good differentiated epidermis after cultured in air-liquid interface.Conclusion The artificial skin made by us is a satisfactory skin substitute with normal dermis and epidermis.The method employed here can be used to fabricate living skin equivalent.