DNA restriction fragment length polymorphism of HLA-DR2: Correlation with HLA-DR2-associated functions |
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| Authors: | Steven Jacobson Rosa Sorrentino Gerald T. Nepom Dale E. McFarlin Jack L. Strominger |
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| Affiliation: | 1. Neuroimmunology Branch, National Institutes of Health, National Institutes of Neurological Communicative Disorders and Stroke, Building 10, Room 5B-16, Bethesda, MD 20205 (U.S.A.);2. Dipartimento di Biologia Cellulare e Dello Sviluppo, Universita di Roma (Italy);3. Genetic SystemsCorporation, Seattle, WA 98121, USA;4. Department of Biochemistry and Molecular Biology, Harvard University,Cambridge, MA 02138 (U.S.A.);1. Laboratory of Histo-Embryology and Cytogenetics, Medicine Faculty of Sfax University, 3029, Sfax, Tunisia;2. Laboratory of Biochemistry, CHU Hédi Chaker of Sfax, 3029, Sfax, Tunisia;3. Laboratory of Histology - Cytology, Medicine Faculty of Tunis, University of Tunis El Manar, 1007, La Rabta-Tunis, Tunisia;1. Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, 517 502, India;2. Department of Zoology, Sri Venkateswara University, Tirupati, 517 502, India;3. Department of Biotechnology, Sri Venkateswara University, Tirupati, 517 502, India;1. School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China;2. School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, China;1. Department of Pulmonary Medicine, Division of Clinical Medicine, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8575, Japan;2. Department of Medical Biochemistry, Tohoku University Graduate School of Medicine, 2-1 Seiryo-cho, Aoba-ku, Sendai, Miyagi 980-8575, Japan;1. Center of Neuroscience Research, Institute of Medical and Health Science of HeBMU, Hebei Medical University, Shijiazhuang, 050017, China;2. Department of Biochemistry and molecular Biology, College of Basic Medicine, Hebei Medical University, Shijiazhuang, 050017, China;3. College of Nursing, Hebei Medical University, Shijiazhuang, 050017, China;4. Experimental Education Center, Clinical college, Hebei Medical University, Shijiazhuang, 050017, China;5. College of Basic Medicine, Hebei Medical University, Shijiazhuang, 050017, China;6. Department of Functional Region of Diagnosis, Fourth Hospital of Hebei Medical University, Hebei Medical University, Shijiazhuang, 050011, China;7. Hebei Key Laboratory of Forensic Medicine, Hebei Medicinal University, 050017, China;8. Collaborative Innovation Center of Forensic Medical Molecular Identification, Hebei Medicinal University, 050017, China;1. Department of Applied Pharmacology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan;2. Department of Orthodontics and Dentofacial Orthopedics, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan;3. Department of Oral Microbiology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan;4. Division of Radiation Biology and Medicine, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Sendai, Japan;5. Burn and Regenerative Medicine Research Center, Velayat Hospital, School of Medicine, Guilan University of Medical Sciences, Parastar St., Rasht, 41887-94755, Iran |
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| Abstract: | HLA-DR2 can be divided into at least 3 distinct HLA-D clusters which correlate with structural differences within the HLA-D region. Further, a functional counterpart of this subdivision has been previously identified. The presence of a particular DR beta 2 polypeptide chain correlated precisely with the susceptibility of measles virus-infected HLA-DR2 homozygous typing cell lines to lysis by measles virus-specific, HLA class II-restricted CTL clones. To determine if a genetic basis for these functional differences could be detected, the degree of polymorphism at the DNA level within the serologically defined HLA-DR2 haplotype has been examined. By using DNA probes for DR beta and DQ beta 4 of the 5 HLA-D clusters of HLA-DR2 could be distinguished and a RFLP pattern was identified which correlates with known immunological functions associated with these various D types. In addition, this technique of 'molecular genotyping' was used to investigate a limited panel of patients with multiple sclerosis (MS) who were HLA typed as either HLA-DR2,2 or HLA-DR2,blank. The RFLP profile of the HLA-DR2 Dw2 D type was found in all of these MS patients. |
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| Keywords: | HLA-DR2 Multiple sclerosis Restriction fragment length polymorphism |
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