Expression and immunohistochemical localization of cathepsin L during the progression of human gliomas |
| |
Authors: | Sivaparvathi Marupudi Yamamoto Masaaki Nicolson Garth L Gokaslan Ziya L Fullert Gregory N Liotta Lance A Sawaya Raymond Rao Jasti S |
| |
Institution: | (1) Department of Neurosurgery, The University of Texas M. D. Anderson Cancer Center, Houston, Texas;(2) Department of Tumor Biology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas;(3) Department of Neuropathology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas;(4) Laboratory of Pathology, National Cancer Institute, Bethesda, MD, USA;(5) Department of Neurosurgery, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Box 064, 77030 Houston, TX, USA |
| |
Abstract: | Recent studies suggest that cysteine proteinase cathepsin L is involved in the process of tumor invasion and metastasis. We
examined cathepsin L activity in brain tumor tissue samples by an enzymatic assay, and cathepsin L protein content by enzyme-linked
immunoadsorbent assays and Western blotting to determine whether increased levels of cathepsin L correlate with the progression
of human gliomas. Native and acid-activatable cathepsin L activities were highest in glioblastomas followed by anaplastic
astrocytomas and were lowest in low-grade gliomas and normal brain tissues. Significantly higher amounts of an M
r
29 000 cathepsin L were present in glioblastomas and anaplastic astrocytomas than in normal brain tissues and low-grade glioma
tissue extracts. Using specific antibodies to cathepsin L, we also studied its cellular distribution by immunohistochemical
procedures. Higher diffuse cathepsin L immunoreactivity was found in glioblastomas than in low-grade gliomas and normal brain
tissue samples. Finally, the addition of cathepsin L antibody inhibits the invasion of glioblastoma cell lines through Matrigel
invasion assay. These results suggest the expression of cathepsin L is dramatically upregulated in malignant gliomas and correlates
with the malignant progression of human gliomas in vivo. |
| |
Keywords: | cathepsins cysteine proteases cysteine protease inhibitors glioblastoma multiforme invasiveness |
本文献已被 SpringerLink 等数据库收录! |
|