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Presence of a linear plasmid-like DNA molecule in the fungal pathogen Ceratocystis fimbriata Ell. & Halst.
Authors:Philippe Normand  Pascal Simonet  Luc Giasson  Patrick Ravel-Chapius  J -André Fortin  Maurice Lalonde
Institution:(1) Laboratoire d'Ecologie Microbienne, CNRS U.A. 697, Université Lyon I, Villeurbanne, 69622 Cedex, France;(2) Départment des Sciences Forestières, Université Lavel, G1K 7P4 Québec, PQ, Canada;(3) Laboratoire de Biologie Cellulaire, CNRS U.A. 92, Université Lyon I, Villeurbanne 69622 Cedex, France
Abstract:Summary A plasmid-like molecule was detected in a strain of the ascomycete Ceratocystis fimbriata Ell. & Halst., a pathogenic fungus of Populus spp. The DNA replicon, designated pFQ501, was found to have a linear structure with a length of 6.0 kb (3.9 × 106 daltons) and a density of 1.685 g/cc. This molecule was found to be associated with the mitochondria and was isolated from the gel; its restriction map was deduced from single and double digestions.
Keywords:Ceratocystis fimbriata  Linear plasmid  mtDNA  Poplar pathogen
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